Oleate dose-dependently regulates palmitate metabolism and insulin signaling in C2C12 myotubes

Because the protective effect of oleate against palmitate-induced insulin resistance may be lessened in skeletal muscle once cell metabolism is overloaded by fatty acids (FAs), we examined the impact of varying amounts of oleate on palmitate metabolic channeling and insulin signaling in C2C12 myotub...

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Bibliographic Details
Published in:Biochimica et biophysica acta 2016-12, Vol.1861 (12), p.2000-2010
Main Authors: Capel, Frédéric, Cheraiti, Naoufel, Acquaviva, Cécile, Hénique, Carole, Bertrand-Michel, Justine, Vianey-Saban, Christine, Prip-Buus, Carina, Morio, Béatrice
Format: Article
Language:English
Subjects:
Animals
Carnitine - analogs & derivatives
Carnitine - metabolism
Cell Line
Ceramides - metabolism
Diglycerides - metabolism
Fatty Acids - metabolism
Food and Nutrition
Insulin - metabolism
Insulin Resistance - physiology
Insulin signaling
Life Sciences
Lipid metabolism
Lipotoxicity
Mice
Muscle Fibers, Skeletal - drug effects
Muscle Fibers, Skeletal - metabolism
Muscle, Skeletal - drug effects
Muscle, Skeletal - metabolism
NF-kappa B - metabolism
Oleic Acid - pharmacology
Oxidation-Reduction - drug effects
p38 Mitogen-Activated Protein Kinases - metabolism
Palmitates - metabolism
Phosphorylation - drug effects
Phosphorylation - physiology
Proto-Oncogene Proteins c-akt - metabolism
Signal Transduction - drug effects
Signal Transduction - physiology
Skeletal muscle
Substrate partitioning
Triglycerides - metabolism
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Summary:Because the protective effect of oleate against palmitate-induced insulin resistance may be lessened in skeletal muscle once cell metabolism is overloaded by fatty acids (FAs), we examined the impact of varying amounts of oleate on palmitate metabolic channeling and insulin signaling in C2C12 myotubes. Cells were exposed to 0.5mM of palmitate and to increasing doses of oleate (0.05, 0.25 and 0.5mM). Impacts of FA treatments on radio-labelled FA fluxes, on cellular content in diacylglycerols (DAG), triacylglycerols (TAG), ceramides, acylcarnitines, on PKCθ, MAPKs (ERK1/2, p38) and NF-ΚB activation, and on insulin-dependent Akt phosphorylation were examined. Low dose of oleate (0.05mM) was sufficient to improve palmitate complete oxidation to CO2 (+29%, P
ISSN:1388-1981
0006-3002
1879-2618
DOI:10.1016/j.bbalip.2016.10.002