Heparan sulfates and the decrease of N-glycans promote early adipogenic differentiation rather than myogenesis of murine myogenic progenitor cells

In vitro, extracted muscle satellite cells, called myogenic progenitor cells, can differentiate either in myotubes or preadipocytes, depending on environmental factors and the medium. Transcriptomic analyses on glycosylation genes during satellite cells differentiation into myotubes showed that 31 g...

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Bibliographic Details
Published in:Differentiation (London) 2017-01, Vol.93, p.15-26
Main Authors: Grassot, Vincent, Bouchatal, Amel, Da Silva, Anne, Chantepie, Sandrine, Papy-Garcia, Dulce, Maftah, Abderrahman, Gallet, Paul-François, Petit, Jean-Michel
Format: Article
Language:English
Subjects:
Adipocytes - cytology
Adipocytes - drug effects
Adipogenesis
Adipogenesis - drug effects
Animals
Carbohydrates
Cell Differentiation - drug effects
Cells
Early adipogenesis
Environmental factors
Glycosylation
Glycosylation related genes
Heparan sulfates
Heparitin Sulfate - administration & dosage
Life Sciences
Mice
Murine myogenic progenitor cells (MPC)
Muscle Cells - cytology
Muscle Cells - drug effects
Muscle Development - drug effects
Muscle Fibers, Skeletal - cytology
Muscle Fibers, Skeletal - drug effects
Myogenesis
Myotubes
N-glycan sialylation
N-glycans
Polysaccharides
Polysaccharides - biosynthesis
Preadipocytes
Progenitor cells
Satellite cells
Sialic acids
Stem cells
Stem Cells - cytology
Stem Cells - drug effects
Transforming growth factor-b1
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Summary:In vitro, extracted muscle satellite cells, called myogenic progenitor cells, can differentiate either in myotubes or preadipocytes, depending on environmental factors and the medium. Transcriptomic analyses on glycosylation genes during satellite cells differentiation into myotubes showed that 31 genes present a significant variation of expression at the early stages of murine myogenic progenitor cells (MPC) differentiation. In the present study, we analyzed the expression of 383 glycosylation related genes during murine MPC differentiation into preadipocytes and compared the data to those previously obtained during their differentiation into myotubes. Fifty-six glycosylation related genes are specifically modified in their expression during early adipogenesis. The variations correspond mainly to: a decrease of N-glycans, and of alpha (2,3) and (2,6) linked sialic acids, and to a high level of heparan sulfates. A high amount of TGF-β1 in extracellular media during early adipogenesis was also observed. It seems that the increases of heparan sulfates and TGF-β1 favor pre-adipogenic differentition of MPC and possibly prevent their myogenic differentiation.
ISSN:0301-4681
1432-0436
DOI:10.1016/j.diff.2016.08.003