Analysis of soil microbial communities based on amplicon sequencing of marker genes

The use of cultivation independent methods has revolutionized soil biology in the last decades. Most popular approaches are based on directly extracted DNA from soil and subsequent analysis of PCR-amplified marker genes by next-generation sequencing. While these high-throughput methods offer novel p...

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Bibliographic Details
Published in:Biology and fertility of soils 2017-07, Vol.53 (5), p.485-489
Main Authors: Schöler, Anne, Jacquiod, Samuel, Vestergaard, Gisle, Schulz, Stefanie, Schloter, Michael
Format: Article
Language:English
Subjects:
Agriculture
Amplification
Biology
Biomedical and Life Sciences
Communities
Cultivation
Data interpretation
Data processing
Deoxyribonucleic acid
DNA
Editorial
Gene sequencing
Genes
Libraries
Life Sciences
Microbial activity
Microorganisms
Polymerase chain reaction
Soil analysis
Soil biology
Soil Science & Conservation
Soils
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Summary:The use of cultivation independent methods has revolutionized soil biology in the last decades. Most popular approaches are based on directly extracted DNA from soil and subsequent analysis of PCR-amplified marker genes by next-generation sequencing. While these high-throughput methods offer novel possibilities over cultivation-based approaches, several key points need to be considered to minimize potential biases during library preparation and downstream bioinformatic analysis. This opinion paper highlights crucial steps that should be considered for accurate analysis and data interpretation.
ISSN:0178-2762
1432-0789
DOI:10.1007/s00374-017-1205-1