Virgibacillus ainsalahensis sp. nov., a Moderately Halophilic Bacterium Isolated from Sediment of a Saline Lake in South of Algeria

• Published in: Current microbiology 2017-02, Vol.74 (2), p.219-223
• Main Authors: Amziane, Meriam, Darenfed-Bouanane, Amel, Abderrahmani, Ahmed, Selama, Okba, Jouadi, Lydia, Cayol, Jean-Luc, Nateche, Farida, Fardeau, Marie-Laure
• Format: Article
• Language: English
• Subjects: Algeria; Bacteria; Bacteriology; Base Composition; Biodiversity; Biomedical and Life Sciences; Biotechnology; Cluster Analysis; Cytosol - chemistry; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; Ecology, environment; Fatty Acids - analysis; Geologic Sediments - microbiology; Glycolipids - analysis; Lakes; Life Sciences; Lipids; Locomotion; Microbiology; Microbiology and Parasitology; Phospholipids - analysis; Phylogeny; RNA, Ribosomal, 16S - genetics; Saline water; Salt lakes; Sediment samplers; Sediments; Sequence Analysis, DNA; Sodium chloride; Sodium Chloride - metabolism; Soil microorganisms; Systematics, Phylogenetics and taxonomy; Temperature; Virgibacillus - classification; Virgibacillus - genetics; Virgibacillus - isolation & purification; Virgibacillus - physiology; Vitamin K 2 - analysis
• ISSN: 0343-8651; 1432-0991
• DOI: 10.1007/s00284-016-1171-0

A Gram-positive, moderately halophilic, endospore-forming bacterium, designated MerV T , was isolated from a sediment sample of a saline lake located in Ain Salah, south of Algeria. The cells were rod shaped and motile. Isolate MerV T grew at salinity interval of 0.5–25% NaCl (optimum, 5–10%), pH 6.0–12.0 (optimum, 8.0), and temperature between 10 and 40 °C (optimum, 30 °C).The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, a glycolipid, a phospholipid, and two lipids, and MK-7 is the predominant menaquinone. The predominant cellular fatty acids were anteiso C 15:0 and anteiso C 17:0 . The DNA G+C content was 45.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain MerV T was most closely related to Virgibacillus halodenitrificans (gene sequence similarity of 97.0%). On the basis of phenotypic, chemotaxonomic properties, and phylogenetic analyses, strain MerV T (=DSM = 28944 T ) should be placed in the genus Virgibacillus as a novel species, for which the name Virgibacillus ainsalahensis is proposed.