PCR Incorporation of Polyoxometalate Modified Deoxynucleotide Triphosphates and Their Application in Molecular Electrochemical Sensing of Yersinia pestis

Redox‐labeled nucleotides are of increasing interest for the fabrication of next generation molecular tools and should meet requirements of being thermally stable, sensitive, and compatible with polymerase‐mediated incorporation while also being electrochemically discriminable. The synthesis and cha...

Full description

Saved in:
Bibliographic Details
Published in:Chemistry : a European journal 2017-08, Vol.23 (44), p.10597-10603
Main Authors: Ortiz, Mayreli, Debela, Ahmed M., Svobodova, Marketa, Thorimbert, Serge, Lesage, Denis, Cole, Richard B., Hasenknopf, Bernold, O'Sullivan, Ciara K.
Format: Article
Language:English
Subjects:
Acetal resins
Amplification
biochemistry
Chemical Sciences
Chemical synthesis
Chemistry
Deoxyribonucleic acid
DNA
DNA, Bacterial - chemistry
DNA, Bacterial - metabolism
Electrochemical Techniques
Electrochemistry
Electrodes
Electrophoresis, Gel, Pulsed-Field
Fabrication
Gold - chemistry
Life Sciences
Nucleotide sequence
Nucleotides
Nucleotides - chemistry
oligonucleotides
Polymerase Chain Reaction
polyoxometalates
Polyoxometallates
Purines
Thermal stability
Tungsten Compounds - chemistry
Yersinia pestis - genetics
Yersinia pestis - isolation & purification
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Redox‐labeled nucleotides are of increasing interest for the fabrication of next generation molecular tools and should meet requirements of being thermally stable, sensitive, and compatible with polymerase‐mediated incorporation while also being electrochemically discriminable. The synthesis and characterization of Keggin and Dawson polyoxometalate–deoxynucleotide (POM–dNTP) bioconjugates linked through 7‐deaza‐modified purines is described. The modified POM–dNTPs were used for polymerase‐based amplification of a DNA sequence specific for Yersinia pestis and the amplified DNA detected using an electrochemical DNA sensor. This highlights the potential of polyoxometalates as thermally stable, sensitive and polymerase‐compatible redox labels for exploitation in bioanalytical applications. Decorating DNA: Keggin and Dawson polyoxometalate–deoxynucleotide (POM–dNTP) bioconjugates were used for decorating a DNA sequence through a polymerase‐based amplification. This amplified DNA product was detected using differential pulse voltammetry.
ISSN:0947-6539
1521-3765
DOI:10.1002/chem.201701295