CHAP domain of Cse functions as an endopeptidase that acts at mature septa to promote Streptococcus thermophilus cell separation

• Published in: Molecular microbiology 2009-03, Vol.71 (5), p.1205-1217
• Main Authors: Layec, Séverine, Gérard, Joëlle, Legué, Valérie, Chapot-Chartier, Marie-Pierre, Courtin, Pascal, Borges, Frédéric, Decaris, Bernard, Leblond-Bourget, Nathalie
• Format: Article
• Language: English
• Subjects: Bacillus subtilis; Bacteria; Bacterial Proteins; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Bacteriology; Biochemistry; Biochemistry, Molecular Biology; Biological and medical sciences; Cell Division; Cell Wall; Cell Wall - enzymology; Cells; Endopeptidases; Endopeptidases - genetics; Endopeptidases - metabolism; Enzyme kinetics; Fluorescence; Fundamental and applied biological sciences. Psychology; Genetic Complementation Test; Life Sciences; Microbiology; Microbiology and Parasitology; Micrococcus lysodeikticus; Miscellaneous; Molecular biology; Mutation; Peptides; Protein Interaction Domains and Motifs; RNA, Bacterial; RNA, Bacterial - genetics; Streptococcus thermophilus; Streptococcus thermophilus - cytology; Streptococcus thermophilus - enzymology; Streptococcus thermophilus - genetics
• ISSN: 0950-382X; 1365-2958
• DOI: 10.1111/j.1365-2958.2009.06595.x

Cell separation is dependent on cell wall hydrolases that cleave the peptidoglycan shared between daughter cells. In Streptococcus thermophilus, this step is performed by the Cse protein whose depletion resulted in the formation of extremely long chains of cells. Cse, a natural chimeric enzyme created by domain shuffling, carries at least two important domains for its activity: the LysM expected to be responsible for the cell wall-binding and the CHAP domain predicted to contain the active centre. Accordingly, the localization of Cse on S. thermophilus cell surface has been undertaken by immunogold electron and immunofluorescence microscopies using of antibodies raised against the N-terminal end of this protein. Immunolocalization shows the presence of the Cse protein at mature septa. Moreover, the CHAP domain of Cse exhibits a cell wall lytic activity in zymograms performed with cell walls of Micrococcus lysodeikticus, Bacillus subtilis and S. thermophilus. Additionally, RP-HPLC analysis of muropeptides released from B. subtilis and S. thermophilus cell wall after digestion with the CHAP domain shows that Cse is an endopeptidase. Altogether, these results suggest that Cse is a cell wall hydrolase involved in daughter cell separation of S. thermophilus.