Ligand- and pH-Induced Conformational Changes of RNA Domain Helix 69 Revealed by 2-Aminopurine Fluorescence

Loop conformation: The loop of the RNA domain helix 69 (H69) was modified with the fluorescent analogue 2‐aminopurine (2AP), thus showing different conformational states under various conditions. The application of this model RNA reveals the unique impact of aminoglycoside neomycin, which differs fr...

Full description

Saved in:
Bibliographic Details
Published in:Angewandte Chemie International Edition 2012-11, Vol.51 (48), p.12095-12098
Main Authors: Sakakibara, Yogo, Abeysirigunawardena, Sanjaya C., Duc, Anne-Cécile E., Dremann, Danielle N., Chow, Christine S.
Format: Article
Language:English
Subjects:
2-Aminopurine - chemistry
antibiotics
Base Sequence
Biochemistry, Molecular Biology
Biophysics
Fluorescent Dyes - chemistry
fluorescent probes
helix 69
Humans
Hydrogen-Ion Concentration
Kinetics
Life Sciences
Ligands
Mass Spectrometry - methods
Nucleic Acid Conformation
ribosome
RNA
RNA, Ribosomal - chemistry
Spectrometry, Fluorescence
Structural Biology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Loop conformation: The loop of the RNA domain helix 69 (H69) was modified with the fluorescent analogue 2‐aminopurine (2AP), thus showing different conformational states under various conditions. The application of this model RNA reveals the unique impact of aminoglycoside neomycin, which differs from the effects of structurally related compounds paromomycin and gentamicin, on the H69 loop conformation in solution (see picture).
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201206000