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Soil prokaryotic communities in Chernobyl waste disposal trench T22 are modulated by organic matter and radionuclide contamination
After the Chernobyl nuclear power plant accident in 1986, contaminated soils, vegetation from the Red Forest and other radioactive debris were buried within trenches. In this area, trench T22 has long been a pilot site for the study of radionuclide migration in soil. Here, we used 454 pyrosequencing...
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| Published in: | FEMS microbiology ecology 2017-08, Vol.93 (8), p.1 |
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| creator | Theodorakopoulos, Nicolas Février, Laureline Barakat, Mohamed Ortet, Philippe Christen, Richard Piette, Laurie Levchuk, Sviatoslav Beaugelin-Seiller, Karine Sergeant, Claire Berthomieu, Catherine Chapon, Virginie |
| description | After the Chernobyl nuclear power plant accident in 1986, contaminated soils, vegetation from the Red Forest and other radioactive debris were buried within trenches. In this area, trench T22 has long been a pilot site for the study of radionuclide migration in soil. Here, we used 454 pyrosequencing of 16S rRNA genes to obtain a comprehensive view of the bacterial and archaeal diversity in soils collected inside and in the vicinity of the trench T22 and to investigate the impact of radioactive waste disposal on prokaryotic communities. A remarkably high abundance of Chloroflexi and AD3 was detected in all soil samples from this area. Our statistical analysis revealed profound changes in community composition at the phylum and OTUs levels and higher diversity in the trench soils as compared to the outside. Our results demonstrate that the total absorbed dose rate by cell and, to a lesser extent the organic matter content of the trench, are the principal variables influencing prokaryotic assemblages. We identified specific phylotypes affiliated to the phyla Crenarchaeota, Acidobacteria, AD3, Chloroflexi, Proteobacteria, Verrucomicrobia and WPS-2, which were unique for the trench soils. |
| doi_str_mv | 10.1093/femsec/fix079 |
| format | article |
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In this area, trench T22 has long been a pilot site for the study of radionuclide migration in soil. Here, we used 454 pyrosequencing of 16S rRNA genes to obtain a comprehensive view of the bacterial and archaeal diversity in soils collected inside and in the vicinity of the trench T22 and to investigate the impact of radioactive waste disposal on prokaryotic communities. A remarkably high abundance of Chloroflexi and AD3 was detected in all soil samples from this area. Our statistical analysis revealed profound changes in community composition at the phylum and OTUs levels and higher diversity in the trench soils as compared to the outside. Our results demonstrate that the total absorbed dose rate by cell and, to a lesser extent the organic matter content of the trench, are the principal variables influencing prokaryotic assemblages. We identified specific phylotypes affiliated to the phyla Crenarchaeota, Acidobacteria, AD3, Chloroflexi, Proteobacteria, Verrucomicrobia and WPS-2, which were unique for the trench soils.</description><identifier>ISSN: 1574-6941</identifier><identifier>ISSN: 0168-6496</identifier><identifier>EISSN: 1574-6941</identifier><identifier>DOI: 10.1093/femsec/fix079</identifier><identifier>PMID: 28645185</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Acidobacteria - classification ; Acidobacteria - genetics ; Acidobacteria - isolation & purification ; Base Sequence ; Biodiversity and Ecology ; Chernobyl Nuclear Accident ; Chloroflexi - classification ; Chloroflexi - genetics ; Chloroflexi - isolation & purification ; Crenarchaeota - classification ; Crenarchaeota - genetics ; Crenarchaeota - isolation & purification ; Environmental aspects ; Environmental Sciences ; Proteobacteria - classification ; Proteobacteria - genetics ; Proteobacteria - isolation & purification ; Radioactive Waste - analysis ; Radioisotopes - analysis ; Refuse Disposal ; RNA, Ribosomal, 16S - genetics ; Sequence Analysis, DNA ; Soil ; Soil Microbiology ; Soil Pollutants, Radioactive - analysis ; Solid Waste - analysis ; Ukraine ; Verrucomicrobia - classification ; Verrucomicrobia - genetics ; Verrucomicrobia - isolation & purification</subject><ispartof>FEMS microbiology ecology, 2017-08, Vol.93 (8), p.1</ispartof><rights>FEMS 2017. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.</rights><rights>COPYRIGHT 2017 Oxford University Press</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c499t-b57c0fde1bd79a84a5e274fe7aeced81fd0f6b2ece3b8ad6e058b41269d0e6923</citedby><cites>FETCH-LOGICAL-c499t-b57c0fde1bd79a84a5e274fe7aeced81fd0f6b2ece3b8ad6e058b41269d0e6923</cites><orcidid>0000-0002-2648-8978 ; 0000-0001-8784-5396 ; 0000-0002-3168-4180 ; 0000-0002-2365-3464 ; 0000-0002-5274-6846</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28645185$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://amu.hal.science/hal-01680959$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Theodorakopoulos, Nicolas</creatorcontrib><creatorcontrib>Février, Laureline</creatorcontrib><creatorcontrib>Barakat, Mohamed</creatorcontrib><creatorcontrib>Ortet, Philippe</creatorcontrib><creatorcontrib>Christen, Richard</creatorcontrib><creatorcontrib>Piette, Laurie</creatorcontrib><creatorcontrib>Levchuk, Sviatoslav</creatorcontrib><creatorcontrib>Beaugelin-Seiller, Karine</creatorcontrib><creatorcontrib>Sergeant, Claire</creatorcontrib><creatorcontrib>Berthomieu, Catherine</creatorcontrib><creatorcontrib>Chapon, Virginie</creatorcontrib><title>Soil prokaryotic communities in Chernobyl waste disposal trench T22 are modulated by organic matter and radionuclide contamination</title><title>FEMS microbiology ecology</title><addtitle>FEMS Microbiol Ecol</addtitle><description>After the Chernobyl nuclear power plant accident in 1986, contaminated soils, vegetation from the Red Forest and other radioactive debris were buried within trenches. 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We identified specific phylotypes affiliated to the phyla Crenarchaeota, Acidobacteria, AD3, Chloroflexi, Proteobacteria, Verrucomicrobia and WPS-2, which were unique for the trench soils.</description><subject>Acidobacteria - classification</subject><subject>Acidobacteria - genetics</subject><subject>Acidobacteria - isolation & purification</subject><subject>Base Sequence</subject><subject>Biodiversity and Ecology</subject><subject>Chernobyl Nuclear Accident</subject><subject>Chloroflexi - classification</subject><subject>Chloroflexi - genetics</subject><subject>Chloroflexi - isolation & purification</subject><subject>Crenarchaeota - classification</subject><subject>Crenarchaeota - genetics</subject><subject>Crenarchaeota - isolation & purification</subject><subject>Environmental aspects</subject><subject>Environmental Sciences</subject><subject>Proteobacteria - classification</subject><subject>Proteobacteria - genetics</subject><subject>Proteobacteria - isolation & purification</subject><subject>Radioactive Waste - analysis</subject><subject>Radioisotopes - analysis</subject><subject>Refuse Disposal</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Sequence Analysis, DNA</subject><subject>Soil</subject><subject>Soil Microbiology</subject><subject>Soil Pollutants, Radioactive - analysis</subject><subject>Solid Waste - analysis</subject><subject>Ukraine</subject><subject>Verrucomicrobia - classification</subject><subject>Verrucomicrobia - genetics</subject><subject>Verrucomicrobia - isolation & purification</subject><issn>1574-6941</issn><issn>0168-6496</issn><issn>1574-6941</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><recordid>eNptkT1vFDEQhlcIREKgpEWWaKDYxN5vl6cTEKSTKAi1NWuPcwZ_HLY3cC2_HJ82RCAhF2O_emY8M29VvWT0klHeXml0CeWVNj_pyB9V56wfu3rgHXv81_2sepbSV0pZ33b0aXXWTEPXs6k_r359DsaSQwzfIB5DNpLI4NziTTaYiPFku8fow3y05AekjESZdAgJLMkRvdyTm6YhEJG4oBYLGRWZjyTEW_ClloOcMRLwikRQJvhFWqOw_OEzOOMhF-159USDTfjiPl5UX96_u9le17tPHz5uN7tadpzneu5HSbVCNquRw9RBj83YaRwBJaqJaUX1MDfl0c4TqAFpP80dawauKA68aS-qt2vdPVhxiMaViUUAI643O3HSKBsmynt-xwr7ZmXLZr4vmLJwJkm0FjyGJQnGWdvyduRDQV-v6C1YFMbrkCPIEy42Y_FkbCjvCnX5H6ochc6UdaA2Rf8noV4TZAwpRdQPLTMqTs6L1XmxOl_4V_cdL7ND9UD_sbr9DQV8rWA</recordid><startdate>20170801</startdate><enddate>20170801</enddate><creator>Theodorakopoulos, Nicolas</creator><creator>Février, Laureline</creator><creator>Barakat, Mohamed</creator><creator>Ortet, Philippe</creator><creator>Christen, Richard</creator><creator>Piette, Laurie</creator><creator>Levchuk, Sviatoslav</creator><creator>Beaugelin-Seiller, Karine</creator><creator>Sergeant, Claire</creator><creator>Berthomieu, Catherine</creator><creator>Chapon, Virginie</creator><general>Oxford University Press</general><general>Wiley-Blackwell</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope><scope>VOOES</scope><orcidid>https://orcid.org/0000-0002-2648-8978</orcidid><orcidid>https://orcid.org/0000-0001-8784-5396</orcidid><orcidid>https://orcid.org/0000-0002-3168-4180</orcidid><orcidid>https://orcid.org/0000-0002-2365-3464</orcidid><orcidid>https://orcid.org/0000-0002-5274-6846</orcidid></search><sort><creationdate>20170801</creationdate><title>Soil prokaryotic communities in Chernobyl waste disposal trench T22 are modulated by organic matter and radionuclide contamination</title><author>Theodorakopoulos, Nicolas ; 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In this area, trench T22 has long been a pilot site for the study of radionuclide migration in soil. Here, we used 454 pyrosequencing of 16S rRNA genes to obtain a comprehensive view of the bacterial and archaeal diversity in soils collected inside and in the vicinity of the trench T22 and to investigate the impact of radioactive waste disposal on prokaryotic communities. A remarkably high abundance of Chloroflexi and AD3 was detected in all soil samples from this area. Our statistical analysis revealed profound changes in community composition at the phylum and OTUs levels and higher diversity in the trench soils as compared to the outside. Our results demonstrate that the total absorbed dose rate by cell and, to a lesser extent the organic matter content of the trench, are the principal variables influencing prokaryotic assemblages. 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| identifier | ISSN: 1574-6941 |
| ispartof | FEMS microbiology ecology, 2017-08, Vol.93 (8), p.1 |
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| language | eng |
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| source | PMC (PubMed Central); Oxford University Press Open Access |
| subjects | Acidobacteria - classification Acidobacteria - genetics Acidobacteria - isolation & purification Base Sequence Biodiversity and Ecology Chernobyl Nuclear Accident Chloroflexi - classification Chloroflexi - genetics Chloroflexi - isolation & purification Crenarchaeota - classification Crenarchaeota - genetics Crenarchaeota - isolation & purification Environmental aspects Environmental Sciences Proteobacteria - classification Proteobacteria - genetics Proteobacteria - isolation & purification Radioactive Waste - analysis Radioisotopes - analysis Refuse Disposal RNA, Ribosomal, 16S - genetics Sequence Analysis, DNA Soil Soil Microbiology Soil Pollutants, Radioactive - analysis Solid Waste - analysis Ukraine Verrucomicrobia - classification Verrucomicrobia - genetics Verrucomicrobia - isolation & purification |
| title | Soil prokaryotic communities in Chernobyl waste disposal trench T22 are modulated by organic matter and radionuclide contamination |
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