Axonal Targeting of the 5‐HT1B Serotonin Receptor Relies on Structure‐Specific Constitutive Activation

• Published in: Traffic (Copenhagen, Denmark) Denmark), 2011-11, Vol.12 (11), p.1501-1520
• Main Authors: Carrel, Damien, Simon, Anne, Emerit, Michel B., Rivals, Isabelle, Leterrier, Christophe, Biard, Marc, Hamon, Michel, Darmon, Michèle, Lenkei, Zsolt
• Format: Article
• Language: English
• Subjects: 7‐transmembrane receptor; Amino Acid Sequence; Animals; axon; Axon guidance; Axons - metabolism; Cell Membrane - metabolism; Cells, Cultured; Cercopithecus aethiops; Chimeras; constitutive activity; COS Cells; Endocytosis; Endocytosis - physiology; G protein-coupled receptors; HeLa Cells; Hippocampus - cytology; Hippocampus - metabolism; Humans; Life Sciences; LLC-PK1 Cells; Molecular Sequence Data; Neurobiology; Neurons; Neurons - metabolism; Neurons and Cognition; p11; Plasma membranes; polarized distribution; Protein Structure, Tertiary; Protein Transport; Rats; Receptor, Serotonin, 5-HT1A - metabolism; Receptor, Serotonin, 5-HT1B - metabolism; Serotonin receptors; Serotonin S1 receptors; Structure-Activity Relationship; Swine; targeting; third intracellular loop; Tumor Cells, Cultured
• ISSN: 1398-9219; 1600-0854
• DOI: 10.1111/j.1600-0854.2011.01260.x

By analogy to other axonal proteins, transcytotic delivery following spontaneous endocytosis from the somatodendritic membrane is expected to be essential for polarized distribution of axonal G‐protein coupled receptors (GPCRs). However, possible contribution from constitutive activation, which may also result in constitutive GPCR endocytosis, is poorly known. Using two closely related but differentially distributed serotonin receptors, here we demonstrate higher constitutive activation and spontaneous endocytosis for the axonal 5‐HT1BR, as compared to the somatodendritic 5‐HT1AR, both in non‐neuronal cells and neurons. Activation‐dependent constitutive endocytosis is crucial for axonal targeting, because inverse‐agonist treatment, which prevents constitutive activation, leads to atypical accumulation of newly synthesized 5‐HT1BRs on the somatodendritic plasma membrane. Using receptor chimeras composed of different domains from 5‐HT1AR and 5‐HT1BR, we show that the complete third intracellular loop of 5‐HT1BR is necessary and sufficient for constitutive activation and efficient axonal targeting, both sensitive to inverse‐agonist treatment. These results suggest that activation and targeting of 5‐HT1BRs are intimately interconnected in neurons.