Light-Induced Nucleation of Metastable Hen Egg-White Lysozyme Solutions

We show through hen egg-white lysozyme nucleation experiments under light produced by a 300 W continuous Xe lamp that nucleation is enhanced. The irradiation of supersaturated protein solutions in NaCl at pH 4.5, β = 9, from 10 to 60 s increased the number of lysozyme crystals in the droplet. The mo...

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Bibliographic Details
Published in:Crystal growth & design 2005-07, Vol.5 (4), p.1393-1398
Main Authors: Okutsu, Tetsuo, Furuta, Kenji, Terao, Motoaki, Hiratsuka, Hiroshi, Yamano, Akihito, Ferté, Natalie, Veesler, Stéphane
Format: Article
Language:English
Subjects:
Condensed Matter
Cross-disciplinary physics: materials science
rheology
Exact sciences and technology
Materials Science
Methods of crystal growth
physics of crystal growth
Physics
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Summary:We show through hen egg-white lysozyme nucleation experiments under light produced by a 300 W continuous Xe lamp that nucleation is enhanced. The irradiation of supersaturated protein solutions in NaCl at pH 4.5, β = 9, from 10 to 60 s increased the number of lysozyme crystals in the droplet. The most effective irradiation time is from 10 to 30 s, and in this range, enzyme activity is not affected. The resulting crystal lattice parameters are identical to that normally obtained. We propose the following mechanism:  intermediates produced by one photon absorption produce lysozyme radicals, which enhance nucleation. These results suggest a new method for controlling nucleation and growth that could be used in structural genomics and the pharmaceutical industry, for instance.
ISSN:1528-7483
1528-7505
DOI:10.1021/cg049604v