Metabolome and proteome changes between biofilm and planktonic phenotypes of the marine bacterium Pseudoalteromonas lipolytica TC8

A number of bacteria adopt various lifestyles such as planktonic free-living or sessile biofilm stages. This enables their survival and development in a wide range of contrasting environments. With the aim of highlighting specific metabolic shifts between these phenotypes and to improve the overall...

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Bibliographic Details
Published in:Biofouling (Chur, Switzerland) Switzerland), 2018-02, Vol.34 (2), p.132-148
Main Authors: Favre, Laurie, Ortalo-Magné, Annick, Pichereaux, Carole, Gargaros, Audrey, Burlet-Schiltz, Odile, Cotelle, Valérie, Culioli, Gérald
Format: Article
Language:English
Subjects:
Analytical chemistry
Arginine
Bacteria
Bacterial Adhesion
Biochemical composition
Biochemistry
Biochemistry, Molecular Biology
Biofilms
Biofouling
Biosynthesis
Cell culture
Cell division
Chemical Sciences
Chromatography, Liquid
Cultures
Genomics
Heme
Histidine
Life Sciences
Lipid composition
Lipids
Liquid chromatography
liquid chromatography-mass spectrometry
Mass spectrometry
Mass spectroscopy
membrane lipids
Membrane proteins
Metabolome
Metabolomics
Mineral nutrients
Nutrient uptake
Ornithine
Peptidases
Phenotype
Phenotypes
Phosphatidylethanolamine
Plankton
Proteins
Proteome
Proteomics
Pseudoalteromonas
Pseudoalteromonas lipolytica TC8
Tandem Mass Spectrometry
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Summary:A number of bacteria adopt various lifestyles such as planktonic free-living or sessile biofilm stages. This enables their survival and development in a wide range of contrasting environments. With the aim of highlighting specific metabolic shifts between these phenotypes and to improve the overall understanding of marine bacterial adhesion, a dual metabolomics/proteomics approach was applied to planktonic and biofilm cultures of the marine bacterium Pseudoalteromonas lipolytica TC8. The liquid chromatography mass spectrometry (LC-MS) based metabolomics study indicated that membrane lipid composition was highly affected by the culture mode: phosphatidylethanolamine (PEs) derivatives were over-produced in sessile cultures while ornithine lipids (OLs) were more specifically synthesized in planktonic samples. In parallel, differences between proteomes revealed that peptidases, oxidases, transcription factors, membrane proteins and the enzymes involved in histidine biosynthesis were over-expressed in biofilms while proteins involved in heme production, nutrient assimilation, cell division and arginine/ornithine biosynthesis were specifically up-regulated in free-living cells.
ISSN:0892-7014
1029-2454
DOI:10.1080/08927014.2017.1413551