Chemical profiling of the tuber of Stephania cambodica Gagnep. (Menispermaceae) and analytical control by UHPLC-DAD

A new aporphine glycoside (1), named 'angkorwatine', and eight known alkaloids: oblongine (2), stepharine (3), asimilobine-β-d-glucopyranoside (4), isocorydine (5), tetrahydropalmatine (THP) (6), jatrorrhizine (7), palmatine (PAL) (8), and roemerine (ROE) (9) were simultaneously isolated f...

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Bibliographic Details
Published in:Natural product research 2017-04, Vol.31 (7), p.802-809
Main Authors: Dary, Chhavarath, Bun, Sok-Siya, Herbette, Gaëtan, Mabrouki, Fathi, Bun, Hot, Kim, Sothea, Jabbour, Florian, Hul, Sovanmoly, Baghdikian, Béatrice, Ollivier, Evelyne
Format: Article
Language:English
Subjects:
Accuracy profile
alkaloid
Alkaloids
Alkaloids - chemistry
Analytical chemistry
Aporphines
Berberine Alkaloids
Chemical Sciences
Chromatography, High Pressure Liquid
Isoquinolines
Life Sciences
Linearity
Magnetic Resonance Spectroscopy
Medicinal plants
Menispermaceae
Menispermaceae - chemistry
method validation
palmatine
Plant Tubers - chemistry
quantification
Reproducibility of Results
roemerine
Selectivity
Spectrometry, Mass, Electrospray Ionization
Stephania - chemistry
tetrahydropalmatine
Tubers
Vegetal Biology
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Summary:A new aporphine glycoside (1), named 'angkorwatine', and eight known alkaloids: oblongine (2), stepharine (3), asimilobine-β-d-glucopyranoside (4), isocorydine (5), tetrahydropalmatine (THP) (6), jatrorrhizine (7), palmatine (PAL) (8), and roemerine (ROE) (9) were simultaneously isolated from the tuber of Stephania cambodica. The development and validation of UHPLC-DAD method was carried out for the quantification of marker compounds (PAL, ROE, THP) of S. cambodica. In addition to good selectivity and linearity (r 2  > 0.997), trueness, precision, and accuracy of the method did not exceed the acceptance limit of ±10% for ROE, THP and ±20% for PAL. Consequently, this method is able to provide accurate results between 1.39-4.18 μg/mL, 2.01-30.72 μg/mL, and 4.29-64.42 μg/mL for PAL, ROE, and THP, respectively. This study shows that the validated UHPLC method is a rapid, innovative and effective analytical approach to control quality of tubers of S. cambodica and to regulate the usage of this plant in traditional medicine.
ISSN:1478-6419
1478-6427
1029-2349
DOI:10.1080/14786419.2016.1247077