Influence of macroporosity on NIH/3T3 adhesion, proliferation, and osteogenic differentiation of MC3T3-E1 over bio-functionalized highly porous titanium implant material

Highly porous Ti implant materials are being used in order to overcome the stress shielding effect on orthopedic implants. However, the lack of bioactivity on Ti surfaces is still a major concern regarding the osseointegration process. It is known that the rapid recruitment of osteoblasts in bone de...

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Bibliographic Details
Published in:Journal of biomedical materials research. Part B, Applied biomaterials Applied biomaterials, 2019-01, Vol.107 (1), p.73-85
Main Authors: Alves, A C, Thibeaux, R, Toptan, F, Pinto, A M P, Ponthiaux, P, David, B
Format: Article
Language:English
Subjects:
Adhesion
Alkaline phosphatase
Animals
Biocompatibility
Bioengineering
Biological activity
Biomaterials
Biomedical materials
Bone healing
Cell Adhesion
Cell Differentiation
Cell Proliferation
Defects
Differentiation (biology)
Fibroblasts
Growth factors
Implants, Experimental
Life Sciences
Macroporosity
Materials research
Materials science
Metallurgical analysis
Metallurgy
Mice
NIH 3T3 Cells
Orthopaedic implants
Osseointegration
Osteoblasts
Osteoblasts - cytology
Osteoblasts - metabolism
Osteogenesis
Oxidation
Porosity
Porous materials
Powder
Powder metallurgy
Recruitment
Repair
Stress shielding
Surgical implants
Titanium
Titanium - chemistry
Transplants & implants
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Summary:Highly porous Ti implant materials are being used in order to overcome the stress shielding effect on orthopedic implants. However, the lack of bioactivity on Ti surfaces is still a major concern regarding the osseointegration process. It is known that the rapid recruitment of osteoblasts in bone defects is an essential prerequisite for efficient bone repair. Conventionally, osteoblast recruitment to bone defects and subsequent bone repair has been achieved using growth factors. Thus, in this study highly porous Ti samples were processed by powder metallurgy using space holder technique followed by the bio-functionalization through microarc oxidation using a Ca- and P-rich electrolyte. The biological response in terms of early cell response, namely, adhesion, spreading, viability, and proliferation of the novel biofunctionalized highly porous Ti was carried out with NIH/3T3 fibroblasts and MC3T3-E1 preosteoblasts in terms of viability, adhesion, proliferation, and alkaline phosphatase activity. Results showed that bio-functionalization did not affect the cell viability. However, bio-functionalized highly porous Ti (22% porosity) enhanced the cell proliferation and activity. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 107B: 73-85, 2019.
ISSN:1552-4973
1552-4981
DOI:10.1002/jbm.b.34096