LIFE BEYOND LIFE – An Easy Way to Derive Lung Fibroblasts from Cadavers

Several protocols have illustrated the possibility of deriving cells, such as fibroblasts, from different organs. These techniques generally concern organs sampled from living persons, but have already been described for cadavers, especially concerning the skin and tendons. We present, for the first...

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Bibliographic Details
Published in:Journal of forensic sciences 2017-09, Vol.62 (5), p.1339-1344
Main Authors: Colomb, Sophie, Bareil, Corinne, Baccino, Eric, Berthet, Jean‐Philippe, Knabe, Lucie, Vachier, Isabelle, Bourdin, Arnaud
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
Cadaver
Cell Culture Techniques
Cells, Cultured
DNA Fingerprinting
explant
Female
fibroblast
Fibroblasts
Fibroblasts - cytology
Fluorescent Antibody Technique
forensic autopsy
Forensic pathology
forensic science
Human health and pathology
Humans
Life Sciences
lung
Lung - cytology
Male
Middle Aged
Multiplex Polymerase Chain Reaction
Organs
Parameter identification
postmortem delay
Regeneration
senescence
Skin
Tendons
Young Adult
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Summary:Several protocols have illustrated the possibility of deriving cells, such as fibroblasts, from different organs. These techniques generally concern organs sampled from living persons, but have already been described for cadavers, especially concerning the skin and tendons. We present, for the first time, an easy way to derive pulmonary fibroblasts from a lung tissue sampled from a cadaver and directly culture plated. The fibroblast output was checked daily. We obtained lung fibroblasts from 3 (60%) cadavers and 2 (100%) living persons. The fibroblast output took about 3 days for cells from living persons and took up to 39 days for those from cadavers. We did not clearly identify any parameters that could explain these differences. Nevertheless, these derived cells had the same features as the source cells, especially in terms of morphology and proliferation, and could potentially be used in different research domains such as forensic or regeneration medicine.
ISSN:0022-1198
1556-4029
DOI:10.1111/1556-4029.13445