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The effect of chlorination and hydrodynamic shear stress on the persistence of bacteriophages associated with drinking water biofilms
Aims This work aimed to assess at pilot scale the effect of chlorination and water flushing on 2‐month‐old drinking water biofilms and, above all, on biofilm‐associated F‐specific RNA bacteriophages MS2, GA and Qβ. Methods and Results Chlorination (4 mg l−1) was applied first with a hydrodynamic she...
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| Published in: | Journal of applied microbiology 2016-10, Vol.121 (4), p.1189-1197 |
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| Main Authors: | , , , , |
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| cited_by | cdi_FETCH-LOGICAL-c4203-1b9c84b418082096443cf7508f2e503e66c02459f383a822fa515db6fa239da3 |
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| cites | cdi_FETCH-LOGICAL-c4203-1b9c84b418082096443cf7508f2e503e66c02459f383a822fa515db6fa239da3 |
| container_end_page | 1197 |
| container_issue | 4 |
| container_start_page | 1189 |
| container_title | Journal of applied microbiology |
| container_volume | 121 |
| creator | Pelleieux, S. Mathieu, L. Block, J.‐C. Gantzer, C. Bertrand, I. |
| description | Aims
This work aimed to assess at pilot scale the effect of chlorination and water flushing on 2‐month‐old drinking water biofilms and, above all, on biofilm‐associated F‐specific RNA bacteriophages MS2, GA and Qβ.
Methods and Results
Chlorination (4 mg l−1) was applied first with a hydrodynamic shear stress of 1 Pa and second with an increase in hydrodynamic shear stress to 10 Pa. Despite a rapid decrease in the number of biofilm bacteria and associated phages, infectious phages were still detected on surfaces after completion of the 150 min cleaning procedure. The resulting sequence of phage removal was: GA > Qβ ≫ MS2.
Conclusions
The effect of chlorine on biofilm bacteria and biofilm‐associated phages was limited to the upper layers of the biofilm and was not enhanced by an increase in hydrodynamic shear stress. A smaller decrease was observed for MS2 than for GA or Qβ after completion of the cleaning procedure.
Significance and Impact of the Study
The differences observed between the three phages suggest that the location of the viral particles in the biofilm, which is related to their surface properties, affects the efficiency of chlorine disinfection. |
| doi_str_mv | 10.1111/jam.13243 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_hal_p</sourceid><recordid>TN_cdi_hal_primary_oai_HAL_hal_01802444v1</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1827899749</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4203-1b9c84b418082096443cf7508f2e503e66c02459f383a822fa515db6fa239da3</originalsourceid><addsrcrecordid>eNqNkctu1DAUhi1ERS-w4AWQJTZ0kdbXJF6OKqCgQWxmbznOceMhiQfb09E8AO-Np1OKhFQJL-yjo8-fjv0j9JaSK1rW9dpMV5QzwV-gM8prWbG6YS8falFJ0rBTdJ7SmhDKiaxfoVPWCMmatjlDv1YDYHAObMbBYTuMIfrZZB9mbOYeD_s-hn4_m8lbnAYwEaccISVcgFzubiAmnzLMFg6CztgM0YfNYO4gYZNSsN5k6PHO5wH3Rf7Dz3d4V3oRdz44P07pNTpxZkzw5vG8QKtPH1c3t9Xy--cvN4tlZQUjvKKdsq3oBG1Jy4iqheDWNZK0joEkHOraEiakcrzlpmXMGUll39XOMK56wy_Q5VE7mFFvop9M3OtgvL5dLPWhR4qZCSHuaWE_HNlNDD-3kLKefLIwjmaGsE2atuUDlWqE-h-USCXKXtD3_6DrsI1zeXOhqGINZaT-O6eNIaUI7mlYSvQhcV0S1w-JF_bdo3HbTdA_kX8iLsD1Edj5EfbPm_TXxbej8jeQa7RV</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1819271206</pqid></control><display><type>article</type><title>The effect of chlorination and hydrodynamic shear stress on the persistence of bacteriophages associated with drinking water biofilms</title><source>Alma/SFX Local Collection</source><creator>Pelleieux, S. ; Mathieu, L. ; Block, J.‐C. ; Gantzer, C. ; Bertrand, I.</creator><creatorcontrib>Pelleieux, S. ; Mathieu, L. ; Block, J.‐C. ; Gantzer, C. ; Bertrand, I.</creatorcontrib><description>Aims
This work aimed to assess at pilot scale the effect of chlorination and water flushing on 2‐month‐old drinking water biofilms and, above all, on biofilm‐associated F‐specific RNA bacteriophages MS2, GA and Qβ.
Methods and Results
Chlorination (4 mg l−1) was applied first with a hydrodynamic shear stress of 1 Pa and second with an increase in hydrodynamic shear stress to 10 Pa. Despite a rapid decrease in the number of biofilm bacteria and associated phages, infectious phages were still detected on surfaces after completion of the 150 min cleaning procedure. The resulting sequence of phage removal was: GA > Qβ ≫ MS2.
Conclusions
The effect of chlorine on biofilm bacteria and biofilm‐associated phages was limited to the upper layers of the biofilm and was not enhanced by an increase in hydrodynamic shear stress. A smaller decrease was observed for MS2 than for GA or Qβ after completion of the cleaning procedure.
Significance and Impact of the Study
The differences observed between the three phages suggest that the location of the viral particles in the biofilm, which is related to their surface properties, affects the efficiency of chlorine disinfection.</description><identifier>ISSN: 1364-5072</identifier><identifier>EISSN: 1365-2672</identifier><identifier>DOI: 10.1111/jam.13243</identifier><identifier>PMID: 27452787</identifier><identifier>CODEN: JAMIFK</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Bacteria ; Bacteriology ; bacteriophage ; Bacteriophages - drug effects ; Bacteriophages - physiology ; biofilm ; Biofilms ; Biofilms - drug effects ; Chlorine - chemistry ; Chlorine - pharmacology ; disinfection ; Disinfection - instrumentation ; Disinfection - methods ; Drinking water ; Drinking Water - chemistry ; Drinking Water - virology ; Halogenation ; Hydrodynamics ; Life Sciences ; Microbiology ; Microbiology and Parasitology ; Shear stress ; Stress, Mechanical ; Virology ; virus</subject><ispartof>Journal of applied microbiology, 2016-10, Vol.121 (4), p.1189-1197</ispartof><rights>2016 The Society for Applied Microbiology</rights><rights>2016 The Society for Applied Microbiology.</rights><rights>Copyright © 2016 The Society for Applied Microbiology</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4203-1b9c84b418082096443cf7508f2e503e66c02459f383a822fa515db6fa239da3</citedby><cites>FETCH-LOGICAL-c4203-1b9c84b418082096443cf7508f2e503e66c02459f383a822fa515db6fa239da3</cites><orcidid>0000-0001-8747-1347 ; 0000-0003-2287-4852 ; 0000-0003-0743-4402</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27452787$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.univ-lorraine.fr/hal-01802444$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Pelleieux, S.</creatorcontrib><creatorcontrib>Mathieu, L.</creatorcontrib><creatorcontrib>Block, J.‐C.</creatorcontrib><creatorcontrib>Gantzer, C.</creatorcontrib><creatorcontrib>Bertrand, I.</creatorcontrib><title>The effect of chlorination and hydrodynamic shear stress on the persistence of bacteriophages associated with drinking water biofilms</title><title>Journal of applied microbiology</title><addtitle>J Appl Microbiol</addtitle><description>Aims
This work aimed to assess at pilot scale the effect of chlorination and water flushing on 2‐month‐old drinking water biofilms and, above all, on biofilm‐associated F‐specific RNA bacteriophages MS2, GA and Qβ.
Methods and Results
Chlorination (4 mg l−1) was applied first with a hydrodynamic shear stress of 1 Pa and second with an increase in hydrodynamic shear stress to 10 Pa. Despite a rapid decrease in the number of biofilm bacteria and associated phages, infectious phages were still detected on surfaces after completion of the 150 min cleaning procedure. The resulting sequence of phage removal was: GA > Qβ ≫ MS2.
Conclusions
The effect of chlorine on biofilm bacteria and biofilm‐associated phages was limited to the upper layers of the biofilm and was not enhanced by an increase in hydrodynamic shear stress. A smaller decrease was observed for MS2 than for GA or Qβ after completion of the cleaning procedure.
Significance and Impact of the Study
The differences observed between the three phages suggest that the location of the viral particles in the biofilm, which is related to their surface properties, affects the efficiency of chlorine disinfection.</description><subject>Bacteria</subject><subject>Bacteriology</subject><subject>bacteriophage</subject><subject>Bacteriophages - drug effects</subject><subject>Bacteriophages - physiology</subject><subject>biofilm</subject><subject>Biofilms</subject><subject>Biofilms - drug effects</subject><subject>Chlorine - chemistry</subject><subject>Chlorine - pharmacology</subject><subject>disinfection</subject><subject>Disinfection - instrumentation</subject><subject>Disinfection - methods</subject><subject>Drinking water</subject><subject>Drinking Water - chemistry</subject><subject>Drinking Water - virology</subject><subject>Halogenation</subject><subject>Hydrodynamics</subject><subject>Life Sciences</subject><subject>Microbiology</subject><subject>Microbiology and Parasitology</subject><subject>Shear stress</subject><subject>Stress, Mechanical</subject><subject>Virology</subject><subject>virus</subject><issn>1364-5072</issn><issn>1365-2672</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><recordid>eNqNkctu1DAUhi1ERS-w4AWQJTZ0kdbXJF6OKqCgQWxmbznOceMhiQfb09E8AO-Np1OKhFQJL-yjo8-fjv0j9JaSK1rW9dpMV5QzwV-gM8prWbG6YS8falFJ0rBTdJ7SmhDKiaxfoVPWCMmatjlDv1YDYHAObMbBYTuMIfrZZB9mbOYeD_s-hn4_m8lbnAYwEaccISVcgFzubiAmnzLMFg6CztgM0YfNYO4gYZNSsN5k6PHO5wH3Rf7Dz3d4V3oRdz44P07pNTpxZkzw5vG8QKtPH1c3t9Xy--cvN4tlZQUjvKKdsq3oBG1Jy4iqheDWNZK0joEkHOraEiakcrzlpmXMGUll39XOMK56wy_Q5VE7mFFvop9M3OtgvL5dLPWhR4qZCSHuaWE_HNlNDD-3kLKefLIwjmaGsE2atuUDlWqE-h-USCXKXtD3_6DrsI1zeXOhqGINZaT-O6eNIaUI7mlYSvQhcV0S1w-JF_bdo3HbTdA_kX8iLsD1Edj5EfbPm_TXxbej8jeQa7RV</recordid><startdate>201610</startdate><enddate>201610</enddate><creator>Pelleieux, S.</creator><creator>Mathieu, L.</creator><creator>Block, J.‐C.</creator><creator>Gantzer, C.</creator><creator>Bertrand, I.</creator><general>Oxford University Press</general><general>Wiley</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TM</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>7U9</scope><scope>H94</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0001-8747-1347</orcidid><orcidid>https://orcid.org/0000-0003-2287-4852</orcidid><orcidid>https://orcid.org/0000-0003-0743-4402</orcidid></search><sort><creationdate>201610</creationdate><title>The effect of chlorination and hydrodynamic shear stress on the persistence of bacteriophages associated with drinking water biofilms</title><author>Pelleieux, S. ; Mathieu, L. ; Block, J.‐C. ; Gantzer, C. ; Bertrand, I.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4203-1b9c84b418082096443cf7508f2e503e66c02459f383a822fa515db6fa239da3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Bacteria</topic><topic>Bacteriology</topic><topic>bacteriophage</topic><topic>Bacteriophages - drug effects</topic><topic>Bacteriophages - physiology</topic><topic>biofilm</topic><topic>Biofilms</topic><topic>Biofilms - drug effects</topic><topic>Chlorine - chemistry</topic><topic>Chlorine - pharmacology</topic><topic>disinfection</topic><topic>Disinfection - instrumentation</topic><topic>Disinfection - methods</topic><topic>Drinking water</topic><topic>Drinking Water - chemistry</topic><topic>Drinking Water - virology</topic><topic>Halogenation</topic><topic>Hydrodynamics</topic><topic>Life Sciences</topic><topic>Microbiology</topic><topic>Microbiology and Parasitology</topic><topic>Shear stress</topic><topic>Stress, Mechanical</topic><topic>Virology</topic><topic>virus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pelleieux, S.</creatorcontrib><creatorcontrib>Mathieu, L.</creatorcontrib><creatorcontrib>Block, J.‐C.</creatorcontrib><creatorcontrib>Gantzer, C.</creatorcontrib><creatorcontrib>Bertrand, I.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Journal of applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pelleieux, S.</au><au>Mathieu, L.</au><au>Block, J.‐C.</au><au>Gantzer, C.</au><au>Bertrand, I.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The effect of chlorination and hydrodynamic shear stress on the persistence of bacteriophages associated with drinking water biofilms</atitle><jtitle>Journal of applied microbiology</jtitle><addtitle>J Appl Microbiol</addtitle><date>2016-10</date><risdate>2016</risdate><volume>121</volume><issue>4</issue><spage>1189</spage><epage>1197</epage><pages>1189-1197</pages><issn>1364-5072</issn><eissn>1365-2672</eissn><coden>JAMIFK</coden><abstract>Aims
This work aimed to assess at pilot scale the effect of chlorination and water flushing on 2‐month‐old drinking water biofilms and, above all, on biofilm‐associated F‐specific RNA bacteriophages MS2, GA and Qβ.
Methods and Results
Chlorination (4 mg l−1) was applied first with a hydrodynamic shear stress of 1 Pa and second with an increase in hydrodynamic shear stress to 10 Pa. Despite a rapid decrease in the number of biofilm bacteria and associated phages, infectious phages were still detected on surfaces after completion of the 150 min cleaning procedure. The resulting sequence of phage removal was: GA > Qβ ≫ MS2.
Conclusions
The effect of chlorine on biofilm bacteria and biofilm‐associated phages was limited to the upper layers of the biofilm and was not enhanced by an increase in hydrodynamic shear stress. A smaller decrease was observed for MS2 than for GA or Qβ after completion of the cleaning procedure.
Significance and Impact of the Study
The differences observed between the three phages suggest that the location of the viral particles in the biofilm, which is related to their surface properties, affects the efficiency of chlorine disinfection.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>27452787</pmid><doi>10.1111/jam.13243</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0001-8747-1347</orcidid><orcidid>https://orcid.org/0000-0003-2287-4852</orcidid><orcidid>https://orcid.org/0000-0003-0743-4402</orcidid></addata></record> |
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| ispartof | Journal of applied microbiology, 2016-10, Vol.121 (4), p.1189-1197 |
| issn | 1364-5072 1365-2672 |
| language | eng |
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| source | Alma/SFX Local Collection |
| subjects | Bacteria Bacteriology bacteriophage Bacteriophages - drug effects Bacteriophages - physiology biofilm Biofilms Biofilms - drug effects Chlorine - chemistry Chlorine - pharmacology disinfection Disinfection - instrumentation Disinfection - methods Drinking water Drinking Water - chemistry Drinking Water - virology Halogenation Hydrodynamics Life Sciences Microbiology Microbiology and Parasitology Shear stress Stress, Mechanical Virology virus |
| title | The effect of chlorination and hydrodynamic shear stress on the persistence of bacteriophages associated with drinking water biofilms |
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