Resting Tension Participates in the Modulation of Active Tension in Isolated Guinea Pig Ventricular Myocytes

• Published in: Journal of molecular and cellular cardiology 1997-06, Vol.29 (6), p.1629-1637
• Main Authors: Cazorla, Olivier, Pascarel, Caroline, Garnier, Didier, Le Guennec, Jean-Yves
• Format: Article
• Language: English
• Subjects: Animals; Calcium - metabolism; Cardiac myocytes; Cardiology and cardiovascular system; Cell Size; Cellular Biology; Excitation–contraction coupling; Frank–Starling law; Guinea Pigs; Heart Ventricles - cytology; Human health and pathology; Length–tension relationship; Life Sciences; Sarcomeres - physiology; Streptomycin - pharmacology; Stress, Mechanical; Stretch; Subcellular Processes
• ISSN: 0022-2828; 1095-8584
• DOI: 10.1006/jmcc.1997.0402

We studied active and passive properties of intact isolated guinea-pig ventricular myocytes in auxotonic conditions. Cells were attached using carbon fibres. The passive properties of the myocytes, in the presence of the stretch-activated channel blocker streptomycin sulphate, could be separated into two groups: stiff cells (stiffness slope=2.88±0.93 nN/μm3,n=63 cells) and compliant cells (stiffness slope=0.91±0.35 nN/μm3,n=52 cells). The study and the localization of the different kind of cells indicated that endocardium is mainly constituted of stiff cells (80%) while the epicardium contained more compliant cells (60%). When a longitudinal strain was applied to compliant cells, an increase in resting tension, diastolic sarcomere length and active tension were observed. On the other hand, in stiff cells, it induced an increase in resting tension and active tension with little change of diastolic sarcomere length. In both kinds of cells, strain had no effect on Ca2+transient amplitude and shape. Plotting active tensionvdiastolic sarcomere length also clearly showed two separated populations of cells, corresponding to stiff and compliant cells. The results of the two groups of cells when plotting active tensionvresting tension could not be distinguished. We conclude that resting tension is an important factor in the modulation of active tension by stretch in addition to interfilament lattice spacing or sarcomere length.