Combinatorial effect of Bacillus thuringiensis kurstaki and Photorhabdus luminescens against Spodoptera littoralis (Lepidoptera: Noctuidae)

Spodoptera littoralis, one of the major pests of many important crop plants, is more susceptible to Bacillus thuringiensis aizawai delta‐endotoxins than to those of Bacillus thuringiensis kurstaki. Within the framework of the development of efficient bioinsecticides and the prevention against insect...

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Bibliographic Details
Published in:Journal of basic microbiology 2014-11, Vol.54 (11), p.1160-1165
Main Authors: Benfarhat-Touzri, Dalel, Amira, Amal Ben, khedher, Saoussen Ben, Givaudan, Alain, Jaoua, Samir, Tounsi, Slim
Format: Article
Language:English
Subjects:
Agricultural sciences
Animals
Bacillus thuringiensis
Bacillus thuringiensis aizawai
Bacillus thuringiensis kurstaki
Bacterial Proteins - toxicity
Biodiversity and Ecology
Biological Assay
Cry toxins
Endotoxins - toxicity
Environmental Sciences
Hemolysin Proteins - toxicity
Lepidoptera
Life Sciences
Noctuidae
Photorhabdus
Photorhabdus - growth & development
Photorhabdus luminescens
Spodoptera - drug effects
Spodoptera - microbiology
Spodoptera - physiology
Spodoptera littoralis
Survival Analysis
Toxicity
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Summary:Spodoptera littoralis, one of the major pests of many important crop plants, is more susceptible to Bacillus thuringiensis aizawai delta‐endotoxins than to those of Bacillus thuringiensis kurstaki. Within the framework of the development of efficient bioinsecticides and the prevention against insect resistance, we tested the effect of mixing B. thuringiensis kurstaki delta‐endotoxins and Photorhabdus luminescens cells on S. littoralis growth. The obtained results showed that the growth inhibition of this insect was more effective when B. thuringiensis kurstaki spore‐crystal mixture and Photorhabdus luminescens cells were used in combination. Furthermore, this synergism is mainly due to the presence of Cry1Ac, which is one of the three delta‐endotoxins that form the crystal of B. thuringiensis kurstaki strain BNS3 in addition to Cry1Aa and Cry2Aa. This work shows a possibility to use B. thuringiensis as a delivery means for Photorhabdus bacteria in order to infect the insect hemocoel and to reduce the risk of developing resistance in the target organism.
ISSN:0233-111X
1521-4028
DOI:10.1002/jobm.201300142