Molecular characterization and prevalence of two capulaviruses: Alfalfa leaf curl virus from France and Euphorbia caput-medusae latent virus from South Africa

Abstract Little is known about the prevalence, diversity, evolutionary processes, genomic structures and population dynamics of viruses in the divergent geminivirus lineage known as the capulaviruses. We determined and analyzed full genome sequences of 13 Euphorbia caput-medusae latent virus (EcmLV)...

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Published in:Virology (New York, N.Y.) N.Y.), 2016-06, Vol.493, p.142-153
Main Authors: Bernardo, Pauline, Muhire, Brejnev, François, Sarah, Deshoux, Maëlle, Hartnady, Penelope, Farkas, Kata, Kraberger, Simona, Filloux, Denis, Fernandez, Emmanuel, Galzi, Serge, Ferdinand, Romain, Granier, Martine, Marais, Armelle, Monge Blasco, Pablo, Candresse, Thierry, Escriu, Fernando, Varsani, Arvind, Harkins, Gordon W, Martin, Darren P, Roumagnac, Philippe
Format: Article
Language:English
Subjects:
Alfalfa leaf curl virus
DNA, Viral
Ecosystem
Euphorbia
Euphorbia - virology
Euphorbia caput-medusae latent virus
France
Geminiviridae
Geminiviridae - classification
Geminiviridae - genetics
Geminiviridae - isolation & purification
Geminiviridae - physiology
Geminivirus
Genome organization
Genome, Viral
Infectious Disease
Life Sciences
Medicago sativa - virology
Nucleic Acid Conformation
Open Reading Frames
Phylogeny
Phytopathology and phytopharmacy
Plant Diseases - virology
Prevalence
Recombination
Recombination, Genetic
Secondary structure
Sequence Analysis, DNA
South Africa
Spain
Vegetal Biology
Virus Latency
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Summary:Abstract Little is known about the prevalence, diversity, evolutionary processes, genomic structures and population dynamics of viruses in the divergent geminivirus lineage known as the capulaviruses. We determined and analyzed full genome sequences of 13 Euphorbia caput-medusae latent virus (EcmLV) and 26 Alfalfa leaf curl virus (ALCV) isolates, and partial genome sequences of 23 EcmLV and 37 ALCV isolates. While EcmLV was asymptomatic in uncultivated southern African Euphorbia caput-medusae , severe alfalfa disease symptoms were associated with ALCV in southern France. The prevalence of both viruses exceeded 10% in their respective hosts. Besides using patterns of detectable negative selection to identify ORFs that are probably functionally expressed, we show that ALCV and EcmLV both display evidence of inter-species recombination and biologically functional genomic secondary structures. Finally, we show that whereas the EcmLV populations likely experience restricted geographical dispersion, ALCV is probably freely moving across the French Mediterranean region.
ISSN:0042-6822
1096-0341
DOI:10.1016/j.virol.2016.03.016