In Vivo Evaluation of Magnetic Targeting in Mice Colon Tumors with Ultra-Magnetic Liposomes Monitored by MRI

• Published in: Molecular imaging and biology 2019-04, Vol.21 (2), p.269-278
• Main Authors: Thébault, Caroline J., Ramniceanu, Grégory, Michel, Aude, Beauvineau, Claire, Girard, Christian, Seguin, Johanne, Mignet, Nathalie, Ménager, Christine, Doan, Bich-Thuy
• Format: Article
• Language: English
• Subjects: Accumulation; Animals; Biochemistry, Molecular Biology; Cancer; Cell Line, Tumor; Cell Survival; Colon; Colonic Neoplasms - diagnostic imaging; Colorectal cancer; Contrast agents; Drug delivery; Drug delivery systems; Efficiency; Evaluation; Female; Image contrast; Imaging; Iron; Life Sciences; Liposomes; Liver - metabolism; Magnetic permeability; Magnetic Resonance Imaging; Magnetics; Magnetite Nanoparticles - chemistry; Magnetite Nanoparticles - ultrastructure; Medical imaging; Medical innovations; Medicine; Medicine & Public Health; Mice; Mice, Inbred BALB C; NIH 3T3 Cells; NMR; Nuclear magnetic resonance; Quantitative analysis; Radiology; Research Article; Tumors
• ISSN: 1536-1632; 1860-2002
• DOI: 10.1007/s11307-018-1238-3

Purpose The development of theranostic nanocarriers as an innovative therapy against cancer has been improved by targeting properties in order to optimize the drug delivery to safely achieve its desired therapeutic effect. The aim of this paper is to evaluate the magnetic targeting (MT) efficiency of ultra-magnetic liposomes (UML) into CT26 murine colon tumor by magnetic resonance imaging (MRI). Procedures Dynamic susceptibility contrast MRI was applied to assess the bloodstream circulation time. A novel semi-quantitative method called % I 0.25 , based on the intensity distribution in T 2 * -weighted MRI images was developed to compare the accumulation of T 2 contrast agent in tumors with or without MT. To evaluate the efficiency of magnetic targeting, the percentage of pixels under the intensity value I 0.25 ( I 0.25  = 0.25( I max  −  I min )) was calculated on the intensity distribution histogram. Results This innovative method of processing MRI images showed the MT efficiency by a % I 0.25 that was significantly higher in tumors using MT compared to passive accumulation, from 15.3 to 28.6 %. This methodology was validated by ex vivo methods with an iron concentration that is 3-fold higher in tumors using MT. Conclusions We have developed a method that allows a semi-quantitative evaluation of targeting efficiency in tumors, which could be applied to different T 2 contrast agents.