Comparison between two derivatization methods of nitrite ion labeled with 15N applied to liquid chromatography-tandem mass spectrometry

The fragmentations of the adducts resulting from the derivatization of nitrite ion by Griess method (formation of an azo compound) and 2,3-diaminonaphthalene (formation of 2,3-naphthotriazole (NAT)) were studied by tandem mass spectrometry (MS/MS). The transition used for quantification was selected...

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Bibliographic Details
Published in:Analytical methods 2018-01, Vol.10 (31), p.3830-3836
Main Authors: Yu, Haiyan, Schmitt, Romain, Sapin, Anne, Chaimbault Patrick, Leroy, Pierre
Format: Article
Language:English
Subjects:
Adducts
Azo compounds
Cardiovascular diseases
Chemical Sciences
Chromatography
Intestine
Liquid chromatography
Mass spectrometry
Mass spectroscopy
Medical treatment
Nitrites
Nitrogen isotopes
Permeability
Scientific imaging
Spectroscopy
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Summary:The fragmentations of the adducts resulting from the derivatization of nitrite ion by Griess method (formation of an azo compound) and 2,3-diaminonaphthalene (formation of 2,3-naphthotriazole (NAT)) were studied by tandem mass spectrometry (MS/MS). The transition used for quantification was selected according to the highest value of the signal-over-blank ratio (S/B) obtained from each adduct fragmentation. When derivatizing nitrite ion labeled with the stable 15N isotope, followed by liquid chromatography (LC)-MS/MS measurement, the lowest limit of quantification obtained was 5 nM with NAT. The method was applied to study the intestinal permeability of S-nitrosoglutathione, a drug candidate for the chronic treatment of cardiovascular disease. This compound was labeled with 15N and its permeability was studied in an ex vivo rat intestine model using an Ussing chamber.
ISSN:1759-9660
1759-9679
DOI:10.1039/c8ay01206g