Uptake of ozone and modification of lipids in Betula Pendula pollen

• Published in: Environmental pollution (1987) 2018-11, Vol.242 (Pt A), p.880-886
• Main Authors: Zhu, Chao, Farah, Jinane, Choël, Marie, Gosselin, Sylvie, Baroudi, Moomen, Petitprez, Denis, Visez, Nicolas
• Format: Article
• Language: English
• Subjects: Betula pendula; Chemical Sciences; Heterogeneous chemistry; Pollen lipid; Uptake coefficient
• ISSN: 0269-7491; 1873-6424
• DOI: 10.1016/j.envpol.2018.07.025

Pollen allergy risk is modified by air pollutants, including ozone, but the chemical modifications induced on pollen grains are poorly understood. Pollen lipidic extract has been shown to act as an adjuvant to the allergenic reaction and therefore, the modification of lipids by air pollutants could have health implications. Birch pollen was exposed in vitro to ozone to explore the reactivity of O3 on its surface and on its lipidic fraction. Uptake coefficients of ozone were determined for ozone concentration of 117 ppb on the surface of native birch pollen (8.6 ± 0.8 × 10−6), defatted pollen (9.9 ± 0.9 × 10−6), and for crushed pollen grains (34±3 × 10−6). The mass of ozone uptaken was increased by a factor of four for crushed pollen compared to native pollen showing a higher susceptibility to ozone of cytoplasmic granules and broken pollen grains. A total mass of extractible lipids of 27 mg per gram of birch pollen was found and a fraction of these lipids was identified and quantified (fatty acids, alkanes, alkenes and aldehydes). The distribution of lipids was modified by ozone exposure of 115 and 1000 ppb for 16 h with the following reactivity: consumption of alkene, formation of aldehydes and formation of nonanoic acid and octadecanoic acid. The quantity of ozone trapped in the lipidic fraction during 15 min at 115 ppb is enough to contribute to the reactivity of one-third of the alkenes demonstrating that pollen could be susceptible to an atmospheric increase of ozone concentration even for a very short duration complicating the understanding of the link between pollen allergy and pollution. [Display omitted] •Extractible lipids of birch pollen are modified by ozone.•Broken pollen grains are much more sensitive to ozone.•High ozone exposure, even for a short duration, can modify lipids. Chemical composition of extractible lipids of birch pollen grains is modified by ozone even for short time exposure. Broken pollen grains are much more susceptible to ozone than intact one.