A new organotypic model containing dermal-type macrophages

:  Human skin equivalents (SEs) are popular three‐dimensional (D) cell culture systems in fundamental and applied dermatology. They have been made to contain dendritic cells, but so far no study on the incorporation of potentially anti‐inflammatory dermal macrophages has been performed. Here, we sho...

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Bibliographic Details
Published in:Experimental dermatology 2011-12, Vol.20 (12), p.1035-1037
Main Authors: Bechetoille, Nicolas, Vachon, Hortense, Gaydon, Amandine, Boher, Aurelie, Fontaine, Thomas, Schaeffer, Evelyne, Decossas, Marion, André-Frei, Valérie, Mueller, Christopher G.
Format: Article
Language:English
Subjects:
Antigens, CD - metabolism
Antigens, Differentiation, Myelomonocytic - metabolism
Biological and medical sciences
CD163 antigen
Cell Adhesion Molecules - metabolism
Cell culture
Cell Culture Techniques - methods
Cell Membrane - metabolism
Cell surface
Cytoplasmic Vesicles - metabolism
DC-SIGN protein
Delta cells
Dendritic cells
Dermatology
Dermis - cytology
fibroblast
Fibroblasts
Fibroblasts - cytology
Histocompatibility antigen HLA
HLA-DR Antigens - metabolism
Humans
Inflammation
Interleukin 10
Interleukin-10 - metabolism
Latex beads
Lectins, C-Type - metabolism
Life Sciences
Lipopolysaccharides
Lipopolysaccharides - pharmacology
macrophage
Macrophages
Macrophages - cytology
Macrophages - drug effects
Macrophages - metabolism
Medical sciences
Microspheres
Monocytes
Monocytes - metabolism
Phagocytosis - physiology
Receptors, Cell Surface - metabolism
scaffolds
Skin
Skin - cytology
skin equivalent
tissue engineering
Tissue Scaffolds
Tumor Necrosis Factor-alpha - metabolism
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Summary::  Human skin equivalents (SEs) are popular three‐dimensional (D) cell culture systems in fundamental and applied dermatology. They have been made to contain dendritic cells, but so far no study on the incorporation of potentially anti‐inflammatory dermal macrophages has been performed. Here, we show that monocyte‐derived dermal‐type macrophages can be introduced into a rigid scaffold with dermal fibroblasts. They maintain their cell surface markers CD163, DC‐SIGN/CD209 and HLA‐DR, which discriminate them from monocytes and dendritic cells. They retain the ability to produce the anti‐inflammatory cytokine IL‐10 in response to lipopolysaccharide (LPS) and to phagocytose latex beads. We thus demonstrate the feasibility of creating macrophage–fibroblast 3D cultures as a first step towards generating SEs with dermal macrophages.
ISSN:0906-6705
1600-0625
DOI:10.1111/j.1600-0625.2011.01383.x