3D Chemical Shift‐Encoded MRI for Volume and Composition Quantification of Abdominal Adipose Tissue During an Overfeeding Protocol in Healthy Volunteers

• Published in: Journal of magnetic resonance imaging 2019-06, Vol.49 (6), p.1587-1599
• Main Authors: Nemeth, Angeline, Segrestin, Bérénice, Leporq, Benjamin, Seyssel, Kevin, Faraz, Khuram, Sauvinet, Valérie, Disse, Emmanuel, Valette, Pierre‐Jean, Laville, Martine, Ratiney, Hélène, Beuf, Olivier
• Format: Article
• Language: English
• Subjects: Adipose tissue; Bioengineering; Body weight; Chemical equilibrium; chemical shift‐encoded imaging; Coding; Composition; Correlation analysis; Correlation coefficient; Correlation coefficients; Dual energy X-ray absorptiometry; Endocrinology and metabolism; fatty acid composition; Fatty acids; Field strength; Gas chromatography; gas chromatography‐mass spectrometry; Human health and pathology; Imaging; in vivo; Life Sciences; Liver; Magnetic resonance imaging; Magnetic resonance spectroscopy; Mass spectrometry; Mass spectroscopy; MR spectroscopy; Organic chemistry; overfeeding; Overweight; Proton density (concentration); Statistical analysis; Statistical methods; Statistical tests; Steam; Vertebrae
• ISSN: 1053-1807; 1522-2586
• DOI: 10.1002/jmri.26532

Background Overweight and obesity are major worldwide health concerns characterized by an abnormal accumulation of fat in adipose tissue (AT) and liver. Purpose To evaluate the volume and the fatty acid (FA) composition of the subcutaneous adipose tissue (SAT) and the visceral adipose tissue (VAT) and the fat content in the liver from 3D chemical‐shift‐encoded (CSE)‐MRI acquisition, before and after a 31‐day overfeeding protocol. Study Type Prospective and longitudinal study. Subjects Twenty‐one nonobese healthy male volunteers. Field Strength/Sequence A 3D spoiled‐gradient multiple echo sequence and STEAM sequence were performed at 3T. Assessment AT volume was automatically segmented on CSE‐MRI between L2 to L4 lumbar vertebrae and compared to the dual‐energy X‐ray absorptiometry (DEXA) measurement. CSE‐MRI and MR spectroscopy (MRS) data were analyzed to assess the proton density fat fraction (PDFF) in the liver and the FA composition in SAT and VAT. Gas chromatography‐mass spectrometry (GC‐MS) analyses were performed on 13 SAT samples as a FA composition countermeasure. Statistical Tests Paired t‐test, Pearson's correlation coefficient, and Bland–Altman plots were used to compare measurements. Results SAT and VAT volumes significantly increased (P