Evaluation of sperm nuclear integrity in patients with different percentages of decapitated sperm in ejaculates

Abstract The decapitated sperm defect is a rare type of teratozoospermia responsible for male infertility. Spermatozoa from patients affected by this syndrome are used for intracytoplasmic sperm injection (ICSI) although little is known about their DNA integrity. This study evaluated sperm nuclear a...

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Bibliographic Details
Published in:Reproductive biomedicine online 2015-07, Vol.31 (1), p.89-99
Main Authors: Rondanino, Christine, Duchesne, Véronique, Escalier, Denise, Jumeau, Fanny, Verhaeghe, France, Peers, Marie-Claire, Mitchell, Valérie, Rives, Nathalie
Format: Article
Language:English
Subjects:
Adult
aneuploidy rates
Cell Nucleus - ultrastructure
chromatin condensation
Chromosome Aberrations
decapitated spermatozoa
DNA Fragmentation
Humans
Infertility, Male - genetics
Infertility, Male - pathology
Life Sciences
Male
Microscopy, Electron, Transmission
Obstetrics and Gynecology
Reproductive Biology
Semen Analysis
Sperm Injections, Intracytoplasmic
Sperm Motility
sperm nucleus
Spermatozoa - physiology
Spermatozoa - ultrastructure
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Summary:Abstract The decapitated sperm defect is a rare type of teratozoospermia responsible for male infertility. Spermatozoa from patients affected by this syndrome are used for intracytoplasmic sperm injection (ICSI) although little is known about their DNA integrity. This study evaluated sperm nuclear alterations in four patients and ten fertile men (control group). Sperm samples were examined by light, transmission electron and high-magnification contrast microscopy and analysed after terminal deoxynucleotidyltransferase-mediated dUTP nick end labelling, aniline blue staining and fluorescence in-situ hybridization. Spermatozoa from patients presented varying degrees of decapitation, along with morphological and ultrastructural head abnormalities. Whereas the proportion of spermatozoa with fragmented DNA and numerical chromosome abnormalities was similar in patients 1–3 and controls, the percentage of spermatozoa with hypocondensed chromatin was higher in patients 1–3 than in fertile men. Patient 4 presented a distinct phenotype, with an increased proportion of flagellated spermatozoa with DNA strand breaks as well as increased aneuploidy and diploidy rates compared with controls and with patients 1–3. No successful pregnancy resulted from ICSI although embryos were obtained for three patients. The morphological defects and the nuclear alterations observed in spermatozoa of patients with the decapitated sperm syndrome may have contributed to ICSI failures.
ISSN:1472-6483
1472-6491
DOI:10.1016/j.rbmo.2015.04.002