The S -locus receptor kinase is inhibited by thioredoxins and activated by pollen coat proteins

The self-incompatibility response in Brassica allows recognition and rejection of self-pollen by the stigmatic papillae. The transmembrane S-locus receptor kinase (SRK), a member of the receptor-like kinase superfamily in plants, mediates recognition of self-pollen on the female side, whereas the S-...

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Bibliographic Details
Published in:Nature (London) 2001-03, Vol.410 (6825), p.220-223
Main Authors: Cock, J. Mark, Dumas, Christian, Gaude, Thierry, Cabrillac, Didier
Format: Article
Language:English
Subjects:
Botanics
Brassica
Enzyme Activation
Enzyme Inhibitors - pharmacology
Enzymes
Fertilization
Flowers & plants
Humanities and Social Sciences
letter
Life Sciences
Molecular Sequence Data
multidisciplinary
Phosphorylation
Plant Proteins - chemistry
Plant Proteins - physiology
Plant reproduction
Plant Structures
Pollen
Pollen - chemistry
Pollen - physiology
Pollination
Protein Kinase Inhibitors
Protein Kinases
Proteins
Recombinant Proteins - genetics
Recombinant Proteins - pharmacology
Science
Science (multidisciplinary)
Signal Transduction
Thioredoxins - genetics
Thioredoxins - pharmacology
Vegetal Biology
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Summary:The self-incompatibility response in Brassica allows recognition and rejection of self-pollen by the stigmatic papillae. The transmembrane S-locus receptor kinase (SRK), a member of the receptor-like kinase superfamily in plants, mediates recognition of self-pollen on the female side, whereas the S-locus cysteine-rich protein (SCR) is the male component of the self-incompatibility response. SCR is presumably located in the pollen coat, and is thought to be the SRK ligand. Although many receptor-like kinases have been isolated in plants, the mechanisms of signal transduction mediated by these molecules remain largely unknown. Here we show that SRK is phosphorylated in vivo within one hour of self-pollination. We also show that, in vitro, autophosphorylation of SRK is prevented by the stigma thioredoxin THL1 in the absence of a ligand. This inhibition is released in a haplotype-specific manner by the addition of pollen coat proteins. Our data indicate that SRK is inhibited by thioredoxins and activated by pollen coat proteins.
ISSN:0028-0836
1476-4687
DOI:10.1038/35065626