Caffeine increases the expression of cystatin SN in human submandibular acinar-like HSG cells

Abstract Objective The study aimed at evaluating in vitro the effect of caffeine on expression of cystatin SN, a potential marker of sensitivity to bitterness in humans. Methods Differentiation of human submandibular gland (HSG) cells was induced by culturing cells on Matrigel. Caffeine cytotoxicity...

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Bibliographic Details
Published in:Archives of oral biology 2013-10, Vol.58 (10), p.1511-1516
Main Authors: Dsamou, Micheline, Morzel, Martine, Le Corre, Ludovic, Séverin, Isabelle, Chagnon, Marie-Christine
Format: Article
Language:English
Subjects:
Advanced Basic Science
Bitterness
Caffeine
Caffeine - pharmacology
Cell Culture Techniques
Collagen
Dentistry
Drug Combinations
Enzyme-Linked Immunosorbent Assay
HSG cell line
Humans
Laminin
Life Sciences
Proteoglycans
Saliva
Salivary Cystatins - biosynthesis
Submandibular gland
Submandibular Gland - cytology
Toxicology
Toxicology and food chain
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Summary:Abstract Objective The study aimed at evaluating in vitro the effect of caffeine on expression of cystatin SN, a potential marker of sensitivity to bitterness in humans. Methods Differentiation of human submandibular gland (HSG) cells was induced by culturing cells on Matrigel. Caffeine cytotoxicity was assessed over 3 days by the Resazurin test. Finally, effects of 5, 50 and 100 μM caffeine exposure on cystatin SN expression were explored over 3 days by ELISA. Results At concentrations relevant to human adult plasma levels (5, 50 and 100 μM), caffeine did not affect cell viability whether cells were differentiated or not. Cystatin SN levels were overall higher in differentiated cells and increased with time in both conditions. There was a significant ( p < 0.001) effect of caffeine on cystatin SN expression specifically in differentiated cells. Conclusions The HSG cell line proved to be a relevant tool to study in vitro the effect of caffeine at concentrations consistent with dietary intake in human subjects. The results suggest that salivary cystatin SN abundance may depend on caffeine intake, with possible consequences on taste sensitivity.
ISSN:0003-9969
1879-1506
DOI:10.1016/j.archoralbio.2013.06.005