Effects of Cyclosporine A in Ex Vivo Reperfused Pig Lungs

Objective Several works highlight the role of CsA in the prevention of IRI, but none focus on isolated lungs. Our objective was to evaluate the effects of CsA on IRI on ex vivo reperfused pig lungs. Methods Thirty‐two pairs of pig lungs were collected and stored for 30 minutes at 4°C. The study was...

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Published in:Microcirculation (New York, N.Y. 1994) N.Y. 1994), 2014-01, Vol.21 (1), p.84-92
Main Authors: Gennai, Stéphane, Souilamas, Redha, Maignan, Maxime, Brouta, Angélique, Pison, Christophe, Fontaine, Eric, Briot, Raphaël
Format: Article
Language:English
Subjects:
Animals
Capillary Permeability - drug effects
Cyclosporine - pharmacology
Cytokines - metabolism
Immunosuppressive Agents - pharmacology
ischemia/reperfusion injury
Life Sciences
Lung Injury - metabolism
Lung Injury - pathology
Lung Injury - physiopathology
lung physiology
lung transplantation
Perfusion
Pulmonary Alveoli - metabolism
Pulmonary Alveoli - pathology
Pulmonary Alveoli - physiopathology
pulmonary function
Pulmonary Gas Exchange - drug effects
Swine
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Summary:Objective Several works highlight the role of CsA in the prevention of IRI, but none focus on isolated lungs. Our objective was to evaluate the effects of CsA on IRI on ex vivo reperfused pig lungs. Methods Thirty‐two pairs of pig lungs were collected and stored for 30 minutes at 4°C. The study was performed in four groups. First, a control group and then three groups receiving different concentrations of CsA (1, 10, and 30 μM) at two different times: once at the moment of lung procurement and another during the reperfusion procedure. The ex vivo lung preparation was set up using an extracorporeal perfusion circuit. Gas exchange parameters, pulmonary hemodynamics, and biological markers of lung injury were collected for the evaluation. Results CsA improved the PaO2/FiO2 ratio, but it also increased PAP, Pcap, and pulmonary vascular resistances with dose‐dependent effects. Lungs treated with high doses of CsA displayed higher capillary‐alveolar permeability to proteins, lower AFC capacities, and elevated concentrations of pro‐inflammatory cytokines. Conclusions These data suggest a possible deleterious imbalance between the beneficial cell properties of CsA in IRI and its hemodynamic effects on microvascularization.
ISSN:1073-9688
1549-8719
DOI:10.1111/micc.12082