Enzymes as Tools in MIP-Sensors

Molecularly imprinted polymers (MIPs) have the potential to complement antibodies in bioanalysis, are more stable under harsh conditions, and are potentially cheaper to produce. However, the affinity and especially the selectivity of MIPs are in general lower than those of their biological pendants....

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Bibliographic Details
Published in:Chemosensors 2017-06, Vol.5 (2), p.11
Main Authors: Yarman, Aysu, Jetzschmann, Katharina, Neumann, Bettina, Zhang, Xiaorong, Wollenberger, Ulla, Cordin, Aude, Haupt, Karsten, Scheller, Frieder
Format: Article
Language:English
Subjects:
Antibodies
Biomimetics
Biotechnology
Chemical Sciences
Enzymes
Imprinted polymers
Life Sciences
Molecular weight
Polymerization
Polymers
Pretreatment
Selectivity
Sensors
Tracers
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Summary:Molecularly imprinted polymers (MIPs) have the potential to complement antibodies in bioanalysis, are more stable under harsh conditions, and are potentially cheaper to produce. However, the affinity and especially the selectivity of MIPs are in general lower than those of their biological pendants. Enzymes are useful tools for the preparation of MIPs for both low and high-molecular weight targets: As a green alternative to the well-established methods of chemical polymerization, enzyme-initiated polymerization has been introduced and the removal of protein templates by proteases has been successfully applied. Furthermore, MIPs have been coupled with enzymes in order to enhance the analytical performance of biomimetic sensors: Enzymes have been used in MIP-sensors as “tracers” for the generation and amplification of the measuring signal. In addition, enzymatic pretreatment of an analyte can extend the analyte spectrum and eliminate interferences.
ISSN:2227-9040
2227-9040
DOI:10.3390/chemosensors5020011