Phorbol ester treatment of HL 60 leukemia cells results in increase of β-(1→4)-galactosyltransferase

• Published in: Carbohydrate research 2000-09, Vol.328 (3), p.301-305
• Main Authors: Pasqualetto, Valérie, Lemaire, Stéphanie, Neel, Dominique, Aubery, Michèle, Berger, Eric G., Derappe, Christian
• Format: Article
• Language: English
• Subjects: Biochemistry, Molecular Biology; Blotting, Northern; Differentiation; Galactosyltransferase; Galactosyltransferases - genetics; Galactosyltransferases - immunology; Galactosyltransferases - metabolism; Glycosylation; HL 60 cells; Humans; Immunoblotting; Life Sciences; Microscopy, Fluorescence; RNA, Messenger - genetics; RNA, Messenger - metabolism; Tetradecanoylphorbol Acetate - pharmacology
• ISSN: 0008-6215; 1873-426X; 0008-6215
• DOI: 10.1016/S0008-6215(00)00116-6

We previously showed that HL 60 leukemia cells exhibit various changes in their cellular glycans after phorbol 12-myristate 13-acetate (PMA) treatment. These changes could originate largely from changes in one or several glycosyltransferases. In this report, we show using enzymatic measures, fluorescence microscopy, immunoblotting and Northern blot that β-(1→4)-galactosyltransferase I (GalT I) activity was higher (>×2) in PMA-treated compared with untreated HL 60 cells. Immunoblotting showed an increased intensity of the GalT I band at 49 kDa and Northern blot a weak increase of the GalT I transcript band, after PMA treatment. Moreover, Northern blot performed after actinomycin-D treatment of the cells, which inhibits transcription, suggests that the observed increase of GalT I expression could originate, in part, from increase of the stability of GalT I transcripts.