A new multiplex PCR assay to distinguish among three cryptic Galba species, intermediate hosts of Fasciola hepatica

•A molecular tool that identifies cryptic freshwater snail species was developed.•This method is accurate, inexpensive, simple, and rapid.•A helpful technic to monitor the invasion of these species.•A helpful technic to develop management strategies with regard to fasciolosis. A molecular tool descr...

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Bibliographic Details
Published in:Veterinary parasitology 2018-02, Vol.251, p.101-105
Main Authors: Alda, Pilar, Lounnas, Manon, Vázquez, Antonio Alejandro, Ayaqui, Rolando, Calvopiña, Manuel, Celi-Erazo, Maritza, Dillon, Robert T., Jarne, Philippe, Loker, Eric S., Muñiz Pareja, Flavia Caroll, Muzzio-Aroca, Jenny, Nárvaez, Alberto Orlando, Noya, Oscar, Robles, Luiggi Martini, Rodríguez-Hidalgo, Richar, Uribe, Nelson, David, Patrice, Pointier, Jean-Pierre, Hurtrez-Boussès, Sylvie
Format: Article
Language:English
Subjects:
Biodiversity
Biotechnology
Fossaria
Infectious disease
Life Sciences
Lymnaea
Lymnaeidae
Microbiology and Parasitology
Microsatellites
Multiplex PCR
Parasitology
Systematics, Phylogenetics and taxonomy
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Summary:•A molecular tool that identifies cryptic freshwater snail species was developed.•This method is accurate, inexpensive, simple, and rapid.•A helpful technic to monitor the invasion of these species.•A helpful technic to develop management strategies with regard to fasciolosis. A molecular tool described here allows in one step for specific discrimination among three cryptic freshwater snail species (genus Galba) involved in fasciolosis transmission, a worldwide infectious disease of humans and livestock. The multiplex PCR approach taken targets for each species a distinctive, known microsatellite locus which is amplified using specific primers designed to generate an amplicon of a distinctive size that can be readily separated from the amplicons of the other two species on an agarose gel. In this way, the three Galba species (G. cubensis, G. schirazensis, and G. truncatula) can be differentiated from one another, including even if DNA from all three were present in the same reaction. The accuracy of this new molecular tool was tested and validated by comparing multiplex PCR results with species identification based on sequences at mitochondrial and nuclear markers. This new method is accurate, inexpensive, simple, rapid, and can be adapted to handle large sample sizes. It will be helpful for monitoring invasion of Galba species and for developing strategies to limit the snail species involved in the emergence or re-emergence of fasciolosis.
ISSN:0304-4017
1873-2550
DOI:10.1016/j.vetpar.2018.01.006