Determination and imaging of metabolites from Vitis vinifera leaves by laser desorption/ionisation time-of-flight mass spectrometry

Analysis of grapevine phytoalexins at the surface of Vitis vinifera leaves has been achieved by laser desorption/ionisation time‐of‐flight mass spectrometry (LDI‐ToFMS) without matrix deposition. This simple and rapid sampling method was successfully applied to map small organic compounds at the sur...

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Bibliographic Details
Published in:Rapid communications in mass spectrometry 2010-02, Vol.24 (3), p.335-342
Main Authors: Hamm, Gregory, Carré, Vincent, Poutaraud, Anne, Maunit, Benoît, Frache, Gilles, Merdinoglu, Didier, Muller, Jean-François
Format: Article
Language:English
Subjects:
Analytical chemistry
Chemical Sciences
Life Sciences
Plant Leaves - chemistry
Plant Leaves - metabolism
Sensitivity and Specificity
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
Stilbenes - analysis
Stilbenes - metabolism
Vegetal Biology
Vitis - chemistry
Vitis - metabolism
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Summary:Analysis of grapevine phytoalexins at the surface of Vitis vinifera leaves has been achieved by laser desorption/ionisation time‐of‐flight mass spectrometry (LDI‐ToFMS) without matrix deposition. This simple and rapid sampling method was successfully applied to map small organic compounds at the surface of grapevine leaves. It was also demonstrated that the laser wavelength is a highly critical parameter. Both 266 and 337 nm laser wavelengths were used but the 266 nm wavelength gave increased spatial resolution and better sensitivity for the detection of the targeted metabolites (resveratrol and linked stilbene compounds). Mass spectrometry imaging of grapevine Cabernet Sauvignon leaves revealed specific locations with respect to Plasmopara viticola pathogen infection or light illumination. Copyright © 2010 John Wiley & Sons, Ltd.
ISSN:0951-4198
1097-0231
DOI:10.1002/rcm.4395