A transcriptome screen in yeast identifies a novel assembly factor for the mitochondrial complex III

Starting from a transcriptome based study of the spatio-temporal expression of yeast genes encoding mitochondrial proteins of unknown function, we have identified the gene BCA1 (YLR077W). A FISH analysis showed that the BCA1 mRNA co-localized with the mitochondrial network. Cellular fractionation re...

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Bibliographic Details
Published in:Mitochondrion 2011-05, Vol.11 (3), p.391-396
Main Authors: Mathieu, Lise, Marsy, Sophie, Saint-Georges, Yann, Jacq, Claude, Dujardin, Geneviève
Format: Article
Language:English
Subjects:
Biochemistry, Molecular Biology
Electron Transport Complex III - metabolism
Gene Expression Profiling
In Situ Hybridization, Fluorescence
Life Sciences
Mitochondrial Membranes - chemistry
Mitochondrial Proteins - genetics
Mitochondrial Proteins - metabolism
Protein Multimerization
Saccharomyces cerevisiae - enzymology
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae Proteins - genetics
Saccharomyces cerevisiae Proteins - metabolism
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Summary:Starting from a transcriptome based study of the spatio-temporal expression of yeast genes encoding mitochondrial proteins of unknown function, we have identified the gene BCA1 (YLR077W). A FISH analysis showed that the BCA1 mRNA co-localized with the mitochondrial network. Cellular fractionation revealed that Bca1 is bound to the mitochondrial inner-membrane and protrudes into the inter-membrane space. We show that Bca1 controls an early step in complex III assembly and that the supra-molecular organization of Bca1 is dependent upon the assembly level of complex III. Thus, Bca1 is a novel assembly factor for the respiratory complex III.
ISSN:1567-7249
1872-8278
DOI:10.1016/j.mito.2010.12.002