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Khaya grandifoliola C.DC: a potential source of active ingredients against hepatitis C virus in vitro
In this study, we examined the antiviral properties of Khaya grandifoliola C.DC (Meliaceae) on the hepatitis C virus (HCV) life cycle in vitro and identified some of the chemical constituents contained in the fraction with the most antiviral activity. Dried bark powder was extracted by maceration in...
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Published in: | Archives of virology 2016-05, Vol.161 (5), p.1169-1181 |
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creator | Galani, Borris Rosnay Tietcheu Sahuc, Marie-Emmanuelle Sass, Gabriele Njayou, Frédéric Nico Loscher, Christine Mkounga, Pierre Deloison, Gaspard Brodin, Priscille Rouillé, Yves Tiegs, Gisa Séron, Karin Moundipa, Paul Fewou |
description | In this study, we examined the antiviral properties of
Khaya grandifoliola
C.DC (Meliaceae) on the hepatitis C virus (HCV) life cycle
in vitro
and identified some of the chemical constituents contained in the fraction with the most antiviral activity. Dried bark powder was extracted by maceration in a methylene chloride/methanol (MCM) system (50:50; v/v) and separated on silica gel by flash chromatography. Infection and replication rates in Huh-7 cells were investigated by luciferase reporter assay and indirect immunofluorescence assay using subgenomic replicons, HCV pseudotyped particles, and cell-culture-derived HCV (HCVcc), respectively. Cell viability was assessed by MTT assay, and cellular gene expression was analysed by qRT-PCR. The chemical composition of the fraction with the most antiviral activity was analysed by coupled gas chromatography and mass spectrometry (GC-MS). Five fractions of different polarities (F0-F100) were obtained from the MCM extract. One fraction (KgF25) showed the strongest antiviral effect on LucUbiNeoET replicons at nontoxic concentrations. Tested at 100 µg/mL, KgF25 had a high inhibitory effect on HCV replication, comparable to that of 0.01 µM daclatasvir or 1 µM telaprevir. This fraction also inhibited HCVcc infection by mostly targeting the entry step. KgF25 inhibited HCV entry in a pan-genotypic manner by directly inactivating free viral particles. Its antiviral effects were mediated by the transcriptional upregulation of the haem oxygenase-1 gene and interferon antiviral response. Three constituents, namely, benzene, 1,1′-(oxydiethylidene)bis (1), carbamic acid, (4-methylphenyl)-, 1-phenyl (2), and 6-phenyl, 4-(1′-oxyethylphenyl) hexene (3), were identified from the active fraction KgF25 by GC-MS.
Khaya grandifoliola
contains ingredients capable of acting on different steps of the HCV life cycle. |
doi_str_mv | 10.1007/s00705-016-2771-5 |
format | article |
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Khaya grandifoliola
C.DC (Meliaceae) on the hepatitis C virus (HCV) life cycle
in vitro
and identified some of the chemical constituents contained in the fraction with the most antiviral activity. Dried bark powder was extracted by maceration in a methylene chloride/methanol (MCM) system (50:50; v/v) and separated on silica gel by flash chromatography. Infection and replication rates in Huh-7 cells were investigated by luciferase reporter assay and indirect immunofluorescence assay using subgenomic replicons, HCV pseudotyped particles, and cell-culture-derived HCV (HCVcc), respectively. Cell viability was assessed by MTT assay, and cellular gene expression was analysed by qRT-PCR. The chemical composition of the fraction with the most antiviral activity was analysed by coupled gas chromatography and mass spectrometry (GC-MS). Five fractions of different polarities (F0-F100) were obtained from the MCM extract. One fraction (KgF25) showed the strongest antiviral effect on LucUbiNeoET replicons at nontoxic concentrations. Tested at 100 µg/mL, KgF25 had a high inhibitory effect on HCV replication, comparable to that of 0.01 µM daclatasvir or 1 µM telaprevir. This fraction also inhibited HCVcc infection by mostly targeting the entry step. KgF25 inhibited HCV entry in a pan-genotypic manner by directly inactivating free viral particles. Its antiviral effects were mediated by the transcriptional upregulation of the haem oxygenase-1 gene and interferon antiviral response. Three constituents, namely, benzene, 1,1′-(oxydiethylidene)bis (1), carbamic acid, (4-methylphenyl)-, 1-phenyl (2), and 6-phenyl, 4-(1′-oxyethylphenyl) hexene (3), were identified from the active fraction KgF25 by GC-MS.
Khaya grandifoliola
contains ingredients capable of acting on different steps of the HCV life cycle.</description><identifier>ISSN: 0304-8608</identifier><identifier>EISSN: 1432-8798</identifier><identifier>DOI: 10.1007/s00705-016-2771-5</identifier><identifier>PMID: 26843184</identifier><language>eng</language><publisher>Vienna: Springer Vienna</publisher><subject>Antiviral Agents - isolation & purification ; Antiviral Agents - pharmacology ; Biochemistry ; Biomedical and Life Sciences ; Biomedicine ; Carcinoma, Hepatocellular - metabolism ; Cell Line, Tumor ; Chromatography ; Chromatography, Gel - methods ; Cytotoxicity ; Dose-Response Relationship, Drug ; FDA approval ; Fluorescent Antibody Technique ; Gas Chromatography-Mass Spectrometry - methods ; Hepacivirus - drug effects ; Hepatitis C ; Hepatitis C virus ; Humans ; Infections ; Infectious Diseases ; Khaya ; Life Sciences ; Liver Neoplasms - metabolism ; Mass spectrometry ; Medical Microbiology ; Meliaceae ; Meliaceae - chemistry ; Microbiology and Parasitology ; Original Article ; Plant Bark - chemistry ; Plant Extracts - isolation & purification ; Plant Extracts - pharmacology ; Scientific imaging ; Virology ; Virus Internalization - drug effects ; Virus Replication - drug effects</subject><ispartof>Archives of virology, 2016-05, Vol.161 (5), p.1169-1181</ispartof><rights>Springer-Verlag Wien 2016</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c439t-e495200aac1433fce5883be441873ceaa658ff84040e8b48fdc619fd9dca830d3</citedby><cites>FETCH-LOGICAL-c439t-e495200aac1433fce5883be441873ceaa658ff84040e8b48fdc619fd9dca830d3</cites><orcidid>0000-0003-0788-9271 ; 0000-0003-0991-7344 ; 0000-0002-9667-133X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26843184$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-02104486$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Galani, Borris Rosnay Tietcheu</creatorcontrib><creatorcontrib>Sahuc, Marie-Emmanuelle</creatorcontrib><creatorcontrib>Sass, Gabriele</creatorcontrib><creatorcontrib>Njayou, Frédéric Nico</creatorcontrib><creatorcontrib>Loscher, Christine</creatorcontrib><creatorcontrib>Mkounga, Pierre</creatorcontrib><creatorcontrib>Deloison, Gaspard</creatorcontrib><creatorcontrib>Brodin, Priscille</creatorcontrib><creatorcontrib>Rouillé, Yves</creatorcontrib><creatorcontrib>Tiegs, Gisa</creatorcontrib><creatorcontrib>Séron, Karin</creatorcontrib><creatorcontrib>Moundipa, Paul Fewou</creatorcontrib><title>Khaya grandifoliola C.DC: a potential source of active ingredients against hepatitis C virus in vitro</title><title>Archives of virology</title><addtitle>Arch Virol</addtitle><addtitle>Arch Virol</addtitle><description>In this study, we examined the antiviral properties of
Khaya grandifoliola
C.DC (Meliaceae) on the hepatitis C virus (HCV) life cycle
in vitro
and identified some of the chemical constituents contained in the fraction with the most antiviral activity. Dried bark powder was extracted by maceration in a methylene chloride/methanol (MCM) system (50:50; v/v) and separated on silica gel by flash chromatography. Infection and replication rates in Huh-7 cells were investigated by luciferase reporter assay and indirect immunofluorescence assay using subgenomic replicons, HCV pseudotyped particles, and cell-culture-derived HCV (HCVcc), respectively. Cell viability was assessed by MTT assay, and cellular gene expression was analysed by qRT-PCR. The chemical composition of the fraction with the most antiviral activity was analysed by coupled gas chromatography and mass spectrometry (GC-MS). Five fractions of different polarities (F0-F100) were obtained from the MCM extract. One fraction (KgF25) showed the strongest antiviral effect on LucUbiNeoET replicons at nontoxic concentrations. Tested at 100 µg/mL, KgF25 had a high inhibitory effect on HCV replication, comparable to that of 0.01 µM daclatasvir or 1 µM telaprevir. This fraction also inhibited HCVcc infection by mostly targeting the entry step. KgF25 inhibited HCV entry in a pan-genotypic manner by directly inactivating free viral particles. Its antiviral effects were mediated by the transcriptional upregulation of the haem oxygenase-1 gene and interferon antiviral response. Three constituents, namely, benzene, 1,1′-(oxydiethylidene)bis (1), carbamic acid, (4-methylphenyl)-, 1-phenyl (2), and 6-phenyl, 4-(1′-oxyethylphenyl) hexene (3), were identified from the active fraction KgF25 by GC-MS.
Khaya grandifoliola
contains ingredients capable of acting on different steps of the HCV life cycle.</description><subject>Antiviral Agents - isolation & purification</subject><subject>Antiviral Agents - pharmacology</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Carcinoma, Hepatocellular - metabolism</subject><subject>Cell Line, Tumor</subject><subject>Chromatography</subject><subject>Chromatography, Gel - methods</subject><subject>Cytotoxicity</subject><subject>Dose-Response Relationship, Drug</subject><subject>FDA approval</subject><subject>Fluorescent Antibody Technique</subject><subject>Gas Chromatography-Mass Spectrometry - methods</subject><subject>Hepacivirus - drug effects</subject><subject>Hepatitis C</subject><subject>Hepatitis C virus</subject><subject>Humans</subject><subject>Infections</subject><subject>Infectious Diseases</subject><subject>Khaya</subject><subject>Life Sciences</subject><subject>Liver Neoplasms - metabolism</subject><subject>Mass spectrometry</subject><subject>Medical Microbiology</subject><subject>Meliaceae</subject><subject>Meliaceae - chemistry</subject><subject>Microbiology and Parasitology</subject><subject>Original Article</subject><subject>Plant Bark - chemistry</subject><subject>Plant Extracts - isolation & purification</subject><subject>Plant Extracts - pharmacology</subject><subject>Scientific imaging</subject><subject>Virology</subject><subject>Virus Internalization - drug effects</subject><subject>Virus Replication - drug effects</subject><issn>0304-8608</issn><issn>1432-8798</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><recordid>eNp1kU1v1DAQhi0EokvhB3BBlriUQ8o4dhKHWxU-iliJC5ytWWey6yobL7azUv89jlIqhMTFtmYev_PxMvZawLUAaN7HfEBVgKiLsmlEUT1hG6FkWeim1U_ZBiSoQtegL9iLGO8AckBWz9lFWWslhVYbRt8OeI98H3Dq3eBH50fk3fXH7gNHfvKJpuRw5NHPwRL3A0eb3Jm4m_aBepfTkeMe3RQTP9AJk0su8o6fXZhjpvIjBf-SPRtwjPTq4b5kPz9_-tHdFtvvX752N9vCKtmmglRblQCINk8hB0uV1nJHSgndSEuIdaWHQStQQHqn9NDbWrRD3_YWtYReXrJ3q-4BR3MK7ojh3nh05vZma5YYlAKU0vVZZPZqZU_B_5opJnN00dI44kR-jkY0yxZLIaqMvv0Hvcv7mPIkC1XKFnJPmRIrZYOPMdDw2IEAs_hlVr9M9sssfplF-c2D8rw7Uv_4449BGShXIObUtKfwV-n_qv4GOBqe0w</recordid><startdate>20160501</startdate><enddate>20160501</enddate><creator>Galani, Borris Rosnay Tietcheu</creator><creator>Sahuc, Marie-Emmanuelle</creator><creator>Sass, Gabriele</creator><creator>Njayou, Frédéric Nico</creator><creator>Loscher, Christine</creator><creator>Mkounga, Pierre</creator><creator>Deloison, Gaspard</creator><creator>Brodin, Priscille</creator><creator>Rouillé, Yves</creator><creator>Tiegs, Gisa</creator><creator>Séron, Karin</creator><creator>Moundipa, Paul Fewou</creator><general>Springer Vienna</general><general>Springer Nature B.V</general><general>Springer Verlag</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0003-0788-9271</orcidid><orcidid>https://orcid.org/0000-0003-0991-7344</orcidid><orcidid>https://orcid.org/0000-0002-9667-133X</orcidid></search><sort><creationdate>20160501</creationdate><title>Khaya grandifoliola C.DC: a potential source of active ingredients against hepatitis C virus in vitro</title><author>Galani, Borris Rosnay Tietcheu ; Sahuc, Marie-Emmanuelle ; Sass, Gabriele ; Njayou, Frédéric Nico ; Loscher, Christine ; Mkounga, Pierre ; Deloison, Gaspard ; Brodin, Priscille ; Rouillé, Yves ; Tiegs, Gisa ; Séron, Karin ; Moundipa, Paul Fewou</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c439t-e495200aac1433fce5883be441873ceaa658ff84040e8b48fdc619fd9dca830d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Antiviral Agents - isolation & purification</topic><topic>Antiviral Agents - pharmacology</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Carcinoma, Hepatocellular - metabolism</topic><topic>Cell Line, Tumor</topic><topic>Chromatography</topic><topic>Chromatography, Gel - methods</topic><topic>Cytotoxicity</topic><topic>Dose-Response Relationship, Drug</topic><topic>FDA approval</topic><topic>Fluorescent Antibody Technique</topic><topic>Gas Chromatography-Mass Spectrometry - methods</topic><topic>Hepacivirus - drug effects</topic><topic>Hepatitis C</topic><topic>Hepatitis C virus</topic><topic>Humans</topic><topic>Infections</topic><topic>Infectious Diseases</topic><topic>Khaya</topic><topic>Life Sciences</topic><topic>Liver Neoplasms - metabolism</topic><topic>Mass spectrometry</topic><topic>Medical Microbiology</topic><topic>Meliaceae</topic><topic>Meliaceae - chemistry</topic><topic>Microbiology and Parasitology</topic><topic>Original Article</topic><topic>Plant Bark - 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Khaya grandifoliola
C.DC (Meliaceae) on the hepatitis C virus (HCV) life cycle
in vitro
and identified some of the chemical constituents contained in the fraction with the most antiviral activity. Dried bark powder was extracted by maceration in a methylene chloride/methanol (MCM) system (50:50; v/v) and separated on silica gel by flash chromatography. Infection and replication rates in Huh-7 cells were investigated by luciferase reporter assay and indirect immunofluorescence assay using subgenomic replicons, HCV pseudotyped particles, and cell-culture-derived HCV (HCVcc), respectively. Cell viability was assessed by MTT assay, and cellular gene expression was analysed by qRT-PCR. The chemical composition of the fraction with the most antiviral activity was analysed by coupled gas chromatography and mass spectrometry (GC-MS). Five fractions of different polarities (F0-F100) were obtained from the MCM extract. One fraction (KgF25) showed the strongest antiviral effect on LucUbiNeoET replicons at nontoxic concentrations. Tested at 100 µg/mL, KgF25 had a high inhibitory effect on HCV replication, comparable to that of 0.01 µM daclatasvir or 1 µM telaprevir. This fraction also inhibited HCVcc infection by mostly targeting the entry step. KgF25 inhibited HCV entry in a pan-genotypic manner by directly inactivating free viral particles. Its antiviral effects were mediated by the transcriptional upregulation of the haem oxygenase-1 gene and interferon antiviral response. Three constituents, namely, benzene, 1,1′-(oxydiethylidene)bis (1), carbamic acid, (4-methylphenyl)-, 1-phenyl (2), and 6-phenyl, 4-(1′-oxyethylphenyl) hexene (3), were identified from the active fraction KgF25 by GC-MS.
Khaya grandifoliola
contains ingredients capable of acting on different steps of the HCV life cycle.</abstract><cop>Vienna</cop><pub>Springer Vienna</pub><pmid>26843184</pmid><doi>10.1007/s00705-016-2771-5</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0003-0788-9271</orcidid><orcidid>https://orcid.org/0000-0003-0991-7344</orcidid><orcidid>https://orcid.org/0000-0002-9667-133X</orcidid></addata></record> |
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language | eng |
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source | Springer Nature |
subjects | Antiviral Agents - isolation & purification Antiviral Agents - pharmacology Biochemistry Biomedical and Life Sciences Biomedicine Carcinoma, Hepatocellular - metabolism Cell Line, Tumor Chromatography Chromatography, Gel - methods Cytotoxicity Dose-Response Relationship, Drug FDA approval Fluorescent Antibody Technique Gas Chromatography-Mass Spectrometry - methods Hepacivirus - drug effects Hepatitis C Hepatitis C virus Humans Infections Infectious Diseases Khaya Life Sciences Liver Neoplasms - metabolism Mass spectrometry Medical Microbiology Meliaceae Meliaceae - chemistry Microbiology and Parasitology Original Article Plant Bark - chemistry Plant Extracts - isolation & purification Plant Extracts - pharmacology Scientific imaging Virology Virus Internalization - drug effects Virus Replication - drug effects |
title | Khaya grandifoliola C.DC: a potential source of active ingredients against hepatitis C virus in vitro |
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