Point-of-care diagnostics for ricin exposure

A long-sought milestone in the defense against bioterrorism is the development of rapid, simple, and near-patient assays for diagnostic and theranostic purposes. Here, we present a powerful test based on a host response to a biological weapon agent, namely the ricin toxin. A signature for exposure t...

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Bibliographic Details
Published in:Lab on a chip 2015-01, Vol.15 (10), p.2308-2317
Main Authors: Diakite, Mohamed Lemine Youba, Rollin, Jérôme, Jary, Dorothée, Berthier, Jean, Mourton-Gilles, Chantal, Sauvaire, Didier, Philippe, Cathy, Delapierre, Guillaume, Gidrol, Xavier
Format: Article
Language:English
Subjects:
Animals
Biological Warfare Agents
Biological weapons
Blood
Cartridges
Diagnostic systems
Exposure
Humans
Lab-On-A-Chip Devices
Life Sciences
Mice
Mice, Inbred BALB C
Microfluidics
Point-of-Care Systems
Ricin - poisoning
Stores
Theranostic Nanomedicine - instrumentation
Theranostic Nanomedicine - methods
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Summary:A long-sought milestone in the defense against bioterrorism is the development of rapid, simple, and near-patient assays for diagnostic and theranostic purposes. Here, we present a powerful test based on a host response to a biological weapon agent, namely the ricin toxin. A signature for exposure to ricin was extracted and characterized in mice and then integrated into a plastic microfluidic cartridge. This enabled early diagnosis of exposure to ricin in mice using a drop of whole blood in less than 1 h and 30 min. The cartridge stores the reagents and implements all of the steps of the analysis, including mRNA extraction from a drop of blood, followed by tens of parallel RT-qPCR reactions. The simple and low-cost microfluidic cartridge developed here may find other applications in point-of-care diagnostics.
ISSN:1473-0197
1473-0189
DOI:10.1039/c5lc00178a