Benzoxazinone kanamycin A conjugate. A new fluorescent probe suitable to detect mycoplasmas in cell culture

Summary— The synthesis of a new benzoxazinone derivative suitable to detect early infection of cultured cells with mycoplasmas is described. p‐[β—(7 — dimethylamino 1,4 — benzoxazin 2 — one 3yl) — vinyl] — phenylpropenoic acid was coupled to kanamycin A, an aminoglycoside leading to a cationic fluor...

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Bibliographic Details
Published in:Biology of the cell 1990, Vol.70 (3), p.101-105
Main Authors: Monsigny, Michel, Midoux, Patrick, Depierreux, Christiane, Bebear, Christine, le Bris, Marie-Thérèse, Valeur, Bernard
Format: Article
Language:English
Subjects:
aminoglycoside
Animals
Benzoxazines
Cell Line
flow cytometry
Fluorescent Dyes
Life Sciences
Microscopy, Fluorescence
Molecular Structure
Mycoplasma - isolation & purification
Oxazines - chemistry
Oxazines - pharmacology
polycation
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Summary:Summary— The synthesis of a new benzoxazinone derivative suitable to detect early infection of cultured cells with mycoplasmas is described. p‐[β—(7 — dimethylamino 1,4 — benzoxazin 2 — one 3yl) — vinyl] — phenylpropenoic acid was coupled to kanamycin A, an aminoglycoside leading to a cationic fluorescent probe which fluoresces at 600 nm upon excitation at 490 nm. This fluorescent probe is shown to heavily label the glycocallix of all the mycoplasma strains tested which are found to be associated with contaminated cultured cells and to allow an easy and rapid detection of contamination by fluorescence microscopy and flow cytometry.
ISSN:0248-4900
1768-322X
DOI:10.1016/0248-4900(90)90365-A