Very high ethanol productivity in an innovative continuous two-stage bioreactor with cell recycle

The performance of an innovative two-stage continuous bioreactor with cell recycle-potentially capable of giving very high ethanol productivity-was investigated. The first stage was dedicated to cell growth, whereas the second stage was dedicated to ethanol production. A high cell density was obtain...

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Bibliographic Details
Published in:Bioprocess and biosystems engineering 2006-06, Vol.29 (1), p.49-57
Main Authors: BEN CHAABANE, F, ALDIGUIER, A. S, ALFENORE, S, CAMELEYRE, X, BLANC, P, BIDEAUX, C, GUILLOUET, S. E, ROUX, G, MOLINA-JOUVE, C
Format: Article
Language:English
Subjects:
Biological and medical sciences
Bioreactors
Bioreactors - microbiology
Biotechnology
Cell Count
Cell Culture Techniques - instrumentation
Cell Culture Techniques - methods
Cell Proliferation
Computer Simulation
Computer-Aided Design
Equipment Design
Equipment Failure Analysis
Ethanol
Ethanol - metabolism
Fundamental and applied biological sciences. Psychology
Glucose - metabolism
Life Sciences
Models, Biological
Saccharomyces cerevisiae
Saccharomyces cerevisiae - physiology
Ultrafiltration
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Summary:The performance of an innovative two-stage continuous bioreactor with cell recycle-potentially capable of giving very high ethanol productivity-was investigated. The first stage was dedicated to cell growth, whereas the second stage was dedicated to ethanol production. A high cell density was obtained by an ultrafiltration module coupled to the outlet of the second reactor. A recycle loop from the second stage to the first one was tested to improve cell viability and activity. Cultivations of Saccharomyces cerevisiae in mineral medium on glucose were performed at 30 degrees Celsius and pH 4. At steady state, total biomass concentrations of 59 and 157 gDCW l(-1) and ethanol concentrations of 31 and 65 g l(-1) were obtained in the first and second stage, respectively. The residual glucose concentration was 73 g l(-1) in the first stage and close to zero in the second stage. The present study shows that a very high ethanol productivity (up to 41 g l(-1) h(-1)) can indeed be obtained with complete conversion of the glucose and with a high ethanol titre (8.3 degrees GL) in the two-stage system.
ISSN:1615-7591
1615-7605
DOI:10.1007/s00449-006-0056-1