The nucleoid as a scaffold for the assembly of bacterial signaling complexes

• Published in: PLoS genetics 2017, Vol.13 (11)
• Main Authors: Moine, Audrey, Espinosa, Leon, Martineau, Eugenie, Yaikhomba, Mutum, Jazleena, P., Byrne, Deborah, Biondi, Emanuele, Notomista, Eugenio, Brilli, Matteo, Molle, Virginie, Gayathri, Pananghat, Mignot, Tam, Mauriello, Emilia
• Format: Article
• Language: English
• Subjects: Bacteriology; Life Sciences; Microbiology and Parasitology
• ISSN: 1553-7390; 1553-7404
• DOI: 10.1371/journal.pgen.1007103

The FrzCD chemoreceptor from the gliding bacterium Myxococcus xanthus forms cyto-plasmic clusters that occupy a large central region of the cell body also occupied by the nucleoid. In this work, we show that FrzCD directly binds to the nucleoid with its N-terminal positively charged tail and recruits active signaling complexes at this location. The FrzCD binding to the nucleoid occur in a DNA-sequence independent manner and leads to the formation of multiple distributed clusters that explore constrained areas. This organization might be required for cooperative interactions between clustered receptors as observed in membrane-bound chemosensory arrays. Author summary In this work, we show that the cytoplasmic chemoreceptor of the Frz chemosensory system , FrzCD, does not bind the cytoplasmic membrane like most MCPs but bind the bacterial nucleoid directly, thus forming distributed protein clusters also containing the FrzE kinase. In vitro and in vivo experiments show that DNA-binding is not sequence-specific and is mediated by a basic aminoacid sequence of the FrzCD N-terminal domain. The deletion of this motif abolishes FrzCD DNA-binding and cooperativity in the response to signals. This work shows the importance of the nucleoid in the organization and functioning of cytoplasmic signaling systems in bacteria.