Simultaneous detection of the protozoan parasites Toxoplasma, Cryptosporidium and Giardia in food matrices and their persistence on basil leaves

Toxoplasma gondii, Cryptosporidium spp. and Giardia intestinalis are emerging pathogen parasites in the food domain. However, without standardized methods for their detection in food matrices, parasitic foodborne outbreaks remain neglected. In this study, a new immunomagnetic separation assay (IMS T...

Full description

Saved in:
Bibliographic Details
Published in:Food microbiology 2016-08, Vol.57, p.36-44
Main Authors: Hohweyer, Jeanne, Cazeaux, Catherine, Travaillé, Emmanuelle, Languet, Emilie, Dumètre, Aurélien, Aubert, Dominique, Terryn, Christine, Dubey, Jitender P., Azas, Nadine, Houssin, Maryline, Loïc, Favennec, Villena, Isabelle, La Carbona, Stéphanie
Format: Article
Language:English
Subjects:
Cryptosporidium
Cryptosporidium - genetics
Cryptosporidium - growth & development
Cryptosporidium - isolation & purification
Food Contamination - analysis
Foods
Fruits
Giardia
Giardia - genetics
Giardia - growth & development
Giardia - isolation & purification
Giardia intestinalis
Immunomagnetic separation
Joining
Life Sciences
Microscopy
Ocimum basilicum
Ocimum basilicum - parasitology
Oocysts - growth & development
Parasites
Persistence
Plant Leaves - parasitology
Protozoa
Real-time qPCR
Rubus - parasitology
Strategy
Toxoplasma - genetics
Toxoplasma - growth & development
Toxoplasma - isolation & purification
Toxoplasma gondii
Vegetables
Viability
Walls
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Toxoplasma gondii, Cryptosporidium spp. and Giardia intestinalis are emerging pathogen parasites in the food domain. However, without standardized methods for their detection in food matrices, parasitic foodborne outbreaks remain neglected. In this study, a new immunomagnetic separation assay (IMS Toxo) targeting the oocyst's wall of T. gondii was developed using a specific purified monoclonal antibody. Performance of this IMS Toxo coupled to microscopic and qPCR analyses was evaluated in terms of limit of detection (LOD) and recovery rate (RR) on: i) simple matrix (LOD = 5 oocysts; RR between 5 and 56%); ii) raspberries and basil (LOD = 33 oocysts/g; RR between 0.2 and 35%). Finally, to simultaneously extract the three protozoa from these food matrices, T. gondii oocysts were directly concentrated (without IMS Toxo) from the supernatant of the IMS of Cryptosporidium and Giardia (oo)cysts. This strategy associated to qPCR detection led to LOD
ISSN:0740-0020
1095-9998
DOI:10.1016/j.fm.2016.01.002