Modelling the competitive growth of Listeria monocytogenes and Listeria innocua in enrichment broths

• Published in: International journal of food microbiology 2002-03, Vol.73 (2), p.261-274
• Main Authors: Cornu, Marie, Kalmokoff, Martin, Flandrois, Jean-Pierre
• Format: Article
• Language: English
• Subjects: Antibiosis; Bacteriocin-like inhibition; Bacteriocins - metabolism; Bacteriology; Biodiversity; Biological and medical sciences; Colony Count, Microbial; Culture Media; Ecology, environment; Ecosystems; Food industries; Food microbiology; Fundamental and applied biological sciences. Psychology; Kinetics; Life Sciences; Listeria - growth & development; Listeria - physiology; Listeria innocua; Listeria monocytogenes; Listeria monocytogenes - growth & development; Microbiology and Parasitology; Overgrowth; Populations and Evolution; Quantitative Methods; Selective advantage; Systematics, Phylogenetics and taxonomy
• ISSN: 0168-1605; 1879-3460
• DOI: 10.1016/S0168-1605(01)00658-4

The overgrowth of Listeria innocua in enrichment broths designed for the isolation of Listeria monocytogenes is believed to result from two factors: a selective growth advantage of L. innocua, and/or an inhibitory interspecies interaction. The generation times of 13 isolates of L. innocua and L. monocytogenes were determined in Brain Heart Infusion (BHI) and a variety of enrichment media. No significant differences were found in growth characteristics between either species in the various media, suggesting that the growth advantage of L. innocua in enrichment media was not as significant as previously described. Kinetic analysis of mixed cultures of L. monocytogenes and isolates of L. innocua producing a variety of inhibitory activities demonstrated the possibility of an inhibitory interaction between these two species resulting in the overgrowth of the enrichment culture with L. innocua. Modelling the evolution of the ratio between two populations in an enrichment process was used to analyze the impact of a selective growth advantage in L. innocua in an enrichment process for growth of L. monocytogenes. These findings support the widely held view that an overgrowth of L. innocua in the enrichment process can result from both a selective growth advantage as well as the production of inhibitory compounds. From a practical perspective, these interactions can result in an increase in false negatives.