Temocillin against Enterobacteriaceae isolates from community-acquired urinary tract infections: low rate of resistance and good accuracy of routine susceptibility testing methods

Abstract Background Temocillin is an old ‘revived’ antibiotic that may play an important role in the treatment of febrile urinary tract infection (UTI). Data regarding its activity against current Enterobacteriaceae isolates as well as the performance of routine susceptibility testing methods are, h...

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Published in:Journal of antimicrobial chemotherapy 2018-07, Vol.73 (7), p.1848-1853
Main Authors: Alexandre, K, Réveillon-Istin, M, Fabre, R, Delbos, V, Etienne, M, Pestel-Caron, M, Dahyot, S, Caron, F
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Bacteriology
Life Sciences
Microbiology and Parasitology
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container_title Journal of antimicrobial chemotherapy
container_volume 73
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Réveillon-Istin, M
Fabre, R
Delbos, V
Etienne, M
Pestel-Caron, M
Dahyot, S
Caron, F
description Abstract Background Temocillin is an old ‘revived’ antibiotic that may play an important role in the treatment of febrile urinary tract infection (UTI). Data regarding its activity against current Enterobacteriaceae isolates as well as the performance of routine susceptibility testing methods are, however, scarce. Objectives To determine the MICs of temocillin for Enterobacteriaceae strains reflecting the current epidemiology and to analyse the accuracy of three commercial methods. Methods Enterobacteriaceae isolates causing community-acquired UTI were prospectively collected from September 2015 to January 2017 in two French centres. Temocillin MIC was determined by agar dilution (AD) as the reference method and then compared with: (i) susceptibility testing by disc diffusion; (ii) MIC determination by Etest; and (iii) MIC estimation by the Vitek 2 automated system. Results A total of 762 Enterobacteriaceae were analysed comprising 658 (86.4%) Escherichia coli and 37 (4.9%) ESBL-producing isolates. Susceptibility rate assessed by AD was 99.6% according to the 8 mg/L clinical breakpoint and was significantly lower against the ESBL-producing isolates than the non-ESBL-producing isolates (94.6% versus 99.9%, P 
doi_str_mv 10.1093/jac/dky101
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Data regarding its activity against current Enterobacteriaceae isolates as well as the performance of routine susceptibility testing methods are, however, scarce. Objectives To determine the MICs of temocillin for Enterobacteriaceae strains reflecting the current epidemiology and to analyse the accuracy of three commercial methods. Methods Enterobacteriaceae isolates causing community-acquired UTI were prospectively collected from September 2015 to January 2017 in two French centres. Temocillin MIC was determined by agar dilution (AD) as the reference method and then compared with: (i) susceptibility testing by disc diffusion; (ii) MIC determination by Etest; and (iii) MIC estimation by the Vitek 2 automated system. Results A total of 762 Enterobacteriaceae were analysed comprising 658 (86.4%) Escherichia coli and 37 (4.9%) ESBL-producing isolates. Susceptibility rate assessed by AD was 99.6% according to the 8 mg/L clinical breakpoint and was significantly lower against the ESBL-producing isolates than the non-ESBL-producing isolates (94.6% versus 99.9%, P &lt; 0.01). The MIC50 and MIC90 for the total set were 3 and 6 mg/L, respectively. According to the 8 mg/L clinical breakpoint, the major error rate was &lt;1% for disc diffusion and Etest, and significantly higher for Vitek 2 (4.3%, P &lt; 0.01), but still low. No very major error was noticed. Conclusions Temocillin showed a high level of activity against Enterobacteriaceae from community-acquired UTI and good to excellent reliability of routine methods for susceptibility testing in such a setting.</description><identifier>ISSN: 0305-7453</identifier><identifier>EISSN: 1460-2091</identifier><identifier>DOI: 10.1093/jac/dky101</identifier><identifier>PMID: 29635629</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Bacteriology ; Life Sciences ; Microbiology and Parasitology</subject><ispartof>Journal of antimicrobial chemotherapy, 2018-07, Vol.73 (7), p.1848-1853</ispartof><rights>The Author(s) 2018. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For permissions, please email: journals.permissions@oup.com. 2018</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c317t-159e0d1739f1307ee5a961eac197305dc118bf3a20429942c5a499a6d3c1d4e93</citedby><cites>FETCH-LOGICAL-c317t-159e0d1739f1307ee5a961eac197305dc118bf3a20429942c5a499a6d3c1d4e93</cites><orcidid>0000-0001-7773-5695 ; 0000-0002-4230-1886</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27922,27923</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29635629$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://normandie-univ.hal.science/hal-02344063$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Alexandre, K</creatorcontrib><creatorcontrib>Réveillon-Istin, M</creatorcontrib><creatorcontrib>Fabre, R</creatorcontrib><creatorcontrib>Delbos, V</creatorcontrib><creatorcontrib>Etienne, M</creatorcontrib><creatorcontrib>Pestel-Caron, M</creatorcontrib><creatorcontrib>Dahyot, S</creatorcontrib><creatorcontrib>Caron, F</creatorcontrib><title>Temocillin against Enterobacteriaceae isolates from community-acquired urinary tract infections: low rate of resistance and good accuracy of routine susceptibility testing methods</title><title>Journal of antimicrobial chemotherapy</title><addtitle>J Antimicrob Chemother</addtitle><description>Abstract Background Temocillin is an old ‘revived’ antibiotic that may play an important role in the treatment of febrile urinary tract infection (UTI). Data regarding its activity against current Enterobacteriaceae isolates as well as the performance of routine susceptibility testing methods are, however, scarce. Objectives To determine the MICs of temocillin for Enterobacteriaceae strains reflecting the current epidemiology and to analyse the accuracy of three commercial methods. Methods Enterobacteriaceae isolates causing community-acquired UTI were prospectively collected from September 2015 to January 2017 in two French centres. Temocillin MIC was determined by agar dilution (AD) as the reference method and then compared with: (i) susceptibility testing by disc diffusion; (ii) MIC determination by Etest; and (iii) MIC estimation by the Vitek 2 automated system. Results A total of 762 Enterobacteriaceae were analysed comprising 658 (86.4%) Escherichia coli and 37 (4.9%) ESBL-producing isolates. Susceptibility rate assessed by AD was 99.6% according to the 8 mg/L clinical breakpoint and was significantly lower against the ESBL-producing isolates than the non-ESBL-producing isolates (94.6% versus 99.9%, P &lt; 0.01). The MIC50 and MIC90 for the total set were 3 and 6 mg/L, respectively. According to the 8 mg/L clinical breakpoint, the major error rate was &lt;1% for disc diffusion and Etest, and significantly higher for Vitek 2 (4.3%, P &lt; 0.01), but still low. No very major error was noticed. Conclusions Temocillin showed a high level of activity against Enterobacteriaceae from community-acquired UTI and good to excellent reliability of routine methods for susceptibility testing in such a setting.</description><subject>Bacteriology</subject><subject>Life Sciences</subject><subject>Microbiology and Parasitology</subject><issn>0305-7453</issn><issn>1460-2091</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp9kctuFDEQRS1ERIbAhg9A3iABUhM_-jFmF0WBRBopm7Bu1djVEwe3PfEDNN_FD-LQIcusSiqfe6vKl5B3nH3hTMnTO9Cn5ueBM_6CrHjbs0YwxV-SFZOsa4a2k8fkdUp3jLG-69evyLFQvex6oVbkzw3OQVvnrKewA-tTphc-Ywxb0LVY0AhIbQoOMiY6xTBTHea5eJsPDej7YiMaWqL1EA80xyqj1k-osw0-faUu_KaxammYaMRkUwavkYI3dBeCoaB1qaLDv_dQsvVIU0ka99luratTaB1c2zs6Y74NJr0hRxO4hG8f6wn58e3i5vyy2Vx_vzo_2zRa8iE3vFPIDB-kmrhkA2IHqucImquhfozRnK-3kwTBWqFUK3QHrVLQG6m5aVHJE_Jp8b0FN-6jneuBYwA7Xp5txoceE7JtWS9_8cp-XNh9DPel7jvOtt7gHHgMJY2CiZbxtRJDRT8vqI4hpYjTkzdn40OgYw10XAKt8PtH37Kd0Tyh_xOswIcFCGX_nNFfKSqthQ</recordid><startdate>20180701</startdate><enddate>20180701</enddate><creator>Alexandre, K</creator><creator>Réveillon-Istin, M</creator><creator>Fabre, R</creator><creator>Delbos, V</creator><creator>Etienne, M</creator><creator>Pestel-Caron, M</creator><creator>Dahyot, S</creator><creator>Caron, F</creator><general>Oxford University Press</general><general>Oxford University Press (OUP)</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0001-7773-5695</orcidid><orcidid>https://orcid.org/0000-0002-4230-1886</orcidid></search><sort><creationdate>20180701</creationdate><title>Temocillin against Enterobacteriaceae isolates from community-acquired urinary tract infections: low rate of resistance and good accuracy of routine susceptibility testing methods</title><author>Alexandre, K ; Réveillon-Istin, M ; Fabre, R ; Delbos, V ; Etienne, M ; Pestel-Caron, M ; Dahyot, S ; Caron, F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c317t-159e0d1739f1307ee5a961eac197305dc118bf3a20429942c5a499a6d3c1d4e93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Bacteriology</topic><topic>Life Sciences</topic><topic>Microbiology and Parasitology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Alexandre, K</creatorcontrib><creatorcontrib>Réveillon-Istin, M</creatorcontrib><creatorcontrib>Fabre, R</creatorcontrib><creatorcontrib>Delbos, V</creatorcontrib><creatorcontrib>Etienne, M</creatorcontrib><creatorcontrib>Pestel-Caron, M</creatorcontrib><creatorcontrib>Dahyot, S</creatorcontrib><creatorcontrib>Caron, F</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Journal of antimicrobial chemotherapy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Alexandre, K</au><au>Réveillon-Istin, M</au><au>Fabre, R</au><au>Delbos, V</au><au>Etienne, M</au><au>Pestel-Caron, M</au><au>Dahyot, S</au><au>Caron, F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Temocillin against Enterobacteriaceae isolates from community-acquired urinary tract infections: low rate of resistance and good accuracy of routine susceptibility testing methods</atitle><jtitle>Journal of antimicrobial chemotherapy</jtitle><addtitle>J Antimicrob Chemother</addtitle><date>2018-07-01</date><risdate>2018</risdate><volume>73</volume><issue>7</issue><spage>1848</spage><epage>1853</epage><pages>1848-1853</pages><issn>0305-7453</issn><eissn>1460-2091</eissn><abstract>Abstract Background Temocillin is an old ‘revived’ antibiotic that may play an important role in the treatment of febrile urinary tract infection (UTI). Data regarding its activity against current Enterobacteriaceae isolates as well as the performance of routine susceptibility testing methods are, however, scarce. Objectives To determine the MICs of temocillin for Enterobacteriaceae strains reflecting the current epidemiology and to analyse the accuracy of three commercial methods. Methods Enterobacteriaceae isolates causing community-acquired UTI were prospectively collected from September 2015 to January 2017 in two French centres. Temocillin MIC was determined by agar dilution (AD) as the reference method and then compared with: (i) susceptibility testing by disc diffusion; (ii) MIC determination by Etest; and (iii) MIC estimation by the Vitek 2 automated system. Results A total of 762 Enterobacteriaceae were analysed comprising 658 (86.4%) Escherichia coli and 37 (4.9%) ESBL-producing isolates. Susceptibility rate assessed by AD was 99.6% according to the 8 mg/L clinical breakpoint and was significantly lower against the ESBL-producing isolates than the non-ESBL-producing isolates (94.6% versus 99.9%, P &lt; 0.01). The MIC50 and MIC90 for the total set were 3 and 6 mg/L, respectively. According to the 8 mg/L clinical breakpoint, the major error rate was &lt;1% for disc diffusion and Etest, and significantly higher for Vitek 2 (4.3%, P &lt; 0.01), but still low. No very major error was noticed. Conclusions Temocillin showed a high level of activity against Enterobacteriaceae from community-acquired UTI and good to excellent reliability of routine methods for susceptibility testing in such a setting.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>29635629</pmid><doi>10.1093/jac/dky101</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0001-7773-5695</orcidid><orcidid>https://orcid.org/0000-0002-4230-1886</orcidid><oa>free_for_read</oa></addata></record>
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title Temocillin against Enterobacteriaceae isolates from community-acquired urinary tract infections: low rate of resistance and good accuracy of routine susceptibility testing methods
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