Deleting a Chromatin Remodeling Gene Increases the Diversity of Secondary Metabolites Produced by Colletotrichum higginsianum

Colletotrichum higginsianum is the causal agent of crucifer anthracnose disease, responsible for important economic losses in Brassica crops. A mutant lacking the CclA subunit of the COMPASS complex was expected to undergo chromatin decondensation and the activation of cryptic secondary metabolite b...

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Bibliographic Details
Published in:Journal of natural products (Washington, D.C.) D.C.), 2019-04, Vol.82 (4), p.813-822
Main Authors: Dallery, Jean-Félix, Le Goff, Géraldine, Adelin, Emilie, Iorga, Bogdan I, Pigné, Sandrine, O’Connell, Richard J, Ouazzani, Jamal
Format: Article
Language:English
Subjects:
Biotechnology
Life Sciences
Microbiology and Parasitology
Mycology
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Summary:Colletotrichum higginsianum is the causal agent of crucifer anthracnose disease, responsible for important economic losses in Brassica crops. A mutant lacking the CclA subunit of the COMPASS complex was expected to undergo chromatin decondensation and the activation of cryptic secondary metabolite biosynthetic gene clusters. Liquid-state fermentation of the ΔcclA mutant coupled with in situ solid-phase extraction led to the production of three families of compounds, namely, colletorin and colletochlorin derivatives with two new representatives, colletorin D (1) and colletorin D acid (2), the diterpenoid α-pyrone higginsianin family with two new analogues, higginsianin C (3) and 13-epi-higginsianin C (4), and sclerosporide (5) coupling a sclerosporin moiety with dimethoxy inositol.
ISSN:0163-3864
1520-6025
DOI:10.1021/acs.jnatprod.8b00796