Imaging thrombosis with (99m)Tc-labeled RAM.1-antibody in vivo

Introduction: Platelets play a major role in thrombo-embolic diseases, notably by forming a thrombus that can ultimately occlude a vessel. This may provoke ischemic pathologies such as myocardial infarction, stroke or peripheral artery diseases, which represent the major causes of death worldwide. T...

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Bibliographic Details
Published in:Nuclear medicine and biology 2018-06, Vol.61, p.21-27
Main Authors: Ouadi, Ali, Bekaert, Virgile, Receveur, Nicolas, Thomas, Lionel, Lanza, François, Marchand, Patrice, Gachet, Christian, Mangin, Pierre Henri, Brasse, David, Laquerriere, Patrice
Format: Article
Language:English
Subjects:
Bioengineering
Cardiology and cardiovascular system
Human health and pathology
Imaging
Life Sciences
Nuclear medicine
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Summary:Introduction: Platelets play a major role in thrombo-embolic diseases, notably by forming a thrombus that can ultimately occlude a vessel. This may provoke ischemic pathologies such as myocardial infarction, stroke or peripheral artery diseases, which represent the major causes of death worldwide. The aim of this study was to evaluate the specificity of radiolabeled Rat-Anti-Mouse antibody (RAM.1).Methods:We describe a method to detect platelets by using a RAM.1 coupled with the chelating agent hydrazinonicotinic acid (HYNIC) conjugated to 99mTc, for Single Photon Emission Computed Tomography (SPECT). To induce platelet accumulation at a site of interest, we used a mouse model of FeCl3 induced injury of the carotid artery. 90 min after i.v. injection of [99mTc][Tc(HYNIC)-RAM.1], biodistribution of the radiolabeled RAM.1 was assessed, SPECT imaging and histological analysis were performed on the mice that underwent FeCl3-induced vessel damage.Results: We demonstrated a quick and strong affinity of the radiolabeled RAM.1 for the platelet thrombus. Results clearly demonstrated the ability of this radioimmunoconjugate for detecting thrombi from 10 min post injection with an exceptional thrombi uptake. Using FeCl3, the median ratio between the thrombus and the background was 12.4 (range 9.3–42.3) as compared to 1.0 (range: 0.86–2.7) p 
ISSN:0969-8051
DOI:10.1016/j.nucmedbio.2018.03.003