Molecular eco-epidemiology on the sympatric Chagas disease vectors Triatoma brasiliensis and Triatoma petrocchiae: Ecotopes, genetic variation, natural infection prevalence by trypanosomatids and parasite genotyping

• Published in: Acta tropica 2020-01, Vol.201, p.105188-105188, Article 105188
• Main Authors: Lima-Oliveira, Tarcianne M., Fontes, Fernanda von Hertwig M., Lilioso, Maurício, Pires-Silva, Dayane, Teixeira, Marta Maria Geraldes, Meza, Jose Gabriel Vergara, Harry, Myriam, Fileé, Jonathan, Costa, Jane, Valença-Barbosa, Carolina, Folly-Ramos, Elaine, Almeida, Carlos Eduardo
• Format: Article
• Language: English
• Subjects: Animals; Biodiversity; Brazil - epidemiology; Chagas Disease - epidemiology; Chagas Disease - transmission; Chagas disease vectors; Demographic events; Ecosystem; Genetic Variation; Genotype; Insect Vectors - parasitology; Life Sciences; Molecular Epidemiology; Populations and Evolution; Prevalence; Sympatry; Triatoma - parasitology; Triatominae; Trypanosoma cruzi - genetics; Trypanosoma infection prevalence
• ISSN: 0001-706X; 1873-6254
• DOI: 10.1016/j.actatropica.2019.105188

Triatoma petrocchiae is the newly member of the Triatoma brasiliensis species complex. This species overlaps with T. brasiliensis in geographic and ecotypic occupation in the sylvatic habitat because both inhabit rocky outcrops in the semi-arid portion of Brazilian northeast. In this region T. brasiliensis is the most important Chagas disease vector because it constantly colonizes domiciles. In contrast, T. petrocchiae is rarely found in peri or intradomiciliary habitats – reason why little is known about this species. Therefore, Here, we present information for the first time on. the T. petrocchiae ecotopes, genetic diversity, Trypanosoma cruzi prevalence/genotyping in comparison to T. brasiliensis. We found T. brasilensis (N = 223) and T. petrocchiae (N = 69) in co-habitation in rocky outcrops in three Districts of Paraíba and Rio Grande do Norte states. Forty-tree T. petrocchiae insects of eleven sampling spots (composing three geographic populations) were genotyped for the mitochondrial Cyt B gene and little geographic structure was observed. Tajima's D test suggested that species is evolving toward a mutation-drift equilibrium in our collection range. Sylvatic T. petrocchiae had 4% (3/68) of infected insects by T. cruzi, whereas T. brasiliensis had 26% (59/223). Fluorescent Fragment Length Barcoding demonstrated that all three T. petrocchiae harbored TcI whereas T. brasiliensis had TcI, but also TcIII, TcII/TcVI and T. rangeli genotype A, sometimes under mixed infections. None of infected T. petrocchiae were carrying mixed infections. However, this result should be confirmed using a larger pool of infected bugs. We here presented the first documentation of T. rangeli infecting T. brasiliensis. The finding of infected T. petrocchiae calls for constant vector monitoring because the epidemiologic scenario is dynamic and sylvatic vectors are progressively found in adaptation to anthropic environments.