Evidence for association of lipopolysaccharide with Pseudomonas fluorescens strain MF0 porin OprF

• Published in: Research in microbiology 2000-12, Vol.151 (10), p.873-876
• Main Authors: Freulet-Marrière, Marie-Anne, El Hamel, Chahrazed, Chevalier, Sylvie, Dé, Emmanuelle, Molle, Gérard, Orange, Nicole
• Format: Article
• Language: English
• Subjects: Bacteriology; Biological and medical sciences; Electrophoresis, Polyacrylamide Gel; Fundamental and applied biological sciences. Psychology; Life Sciences; lipopolysaccharide; Lipopolysaccharides - isolation & purification; Lipopolysaccharides - metabolism; Microbiology; Microbiology and Parasitology; Morphology, structure, chemical composition; OprF protein; porin OprF; Porins - isolation & purification; Porins - metabolism; Pseudomonas fluorescens; Pseudomonas fluorescens - growth & development; Pseudomonas fluorescens - metabolism; psychrotrophy; Temperature
• ISSN: 0923-2508; 1769-7123; 0923-2508
• DOI: 10.1016/S0923-2508(00)01154-2

Lipopolysaccharide (LPS) was found to be associated with the major outer membrane protein OprF of the psychrotrophic bacterium Pseudomonas fluorescens MF0, using two OprF purification procedures. OprF, purified under mild conditions, presented two types of association with LPS: tight (tLPS) and slight (sLPS), both of type R. LPS protected OprF from heat modification and trypsin degradation and facilitated the reincorporation of purified OprF into an artificial lipid bilayer without affecting its pore-forming activity. The size of the OprF channel depended on cell growth temperature, as did the extent of LPS phosphorylation: we suggest that LPS may be involved in modifications of OprF pore formation.