A Double-Pulse Approach For Electrotransfection

• Published in: The Journal of membrane biology 2014-12, Vol.247 (12), p.1253-1258
• Main Authors: Pasquet, L., Bellard, E., Golzio, M., Rols, M. P., Teissie, J.
• Format: Article
• Language: English
• Subjects: Animals; Biochemistry; Biomedical and Life Sciences; Cancer; Cell Line; Cell Membrane - metabolism; Cell Membrane - physiology; Cellular biology; CHO Cells; Cricetulus; Electric fields; Electricity; Electroporation - methods; Green Fluorescent Proteins - metabolism; Human Physiology; Life Sciences; Membranes; Nuclear Envelope - metabolism; Nuclear Envelope - physiology; Plasmids; Plasmids - metabolism; Transfection - methods
• ISSN: 0022-2631; 1432-1424
• DOI: 10.1007/s00232-014-9720-6

Gene transfer and expression can be obtained by delivering calibrated electric pulses on cells in the presence of plasmids coding for the activity of interest. The electric treatment affects the plasma membrane and induces the formation of a transient complex between nucleic acids and the plasma membrane. It results in a delivery of the plasmid in the cytoplasm. Expression is only obtained if the plasmid is translocated inside the nucleus. This is a key limit in the process. We previously showed that delivery of a high-field short-duration electric pulse was inducing a structural alteration of the nuclear envelope. This study investigates if the double-pulse approach (first pulse to transfer the plasmid to the cytoplasm, and second pulse to induce the structural alteration of the envelope) was a way to enhance the protein expression using the green fluorescent protein as a reporter. We observed that not only the double-pulse approach induced the transfection of a lower number of cells but moreover, these transfected cells were less fluorescent than the cells treated only with the first pulse.