A systems biology view of wood formation in Eucalyptus grandis trees submitted to different potassium and water regimes

• Published in: The New phytologist 2019-07, Vol.223 (2), p.766-782
• Main Authors: Ployet, Raphael, Labate, Mônica T. Veneziano, Cataldi, Thais Regiani, Christina, Mathias, Morel, Marie, San Clemente, Haélène, Denis, Marie, Favreau, Bénédicte, Filho, Mario Tomazello, Laclau, Jean-Paul, Labate, Carlos Alberto, Chaix, Gilles, Grima-Pettenati, Jacqueline, Mounet, Fabien
• Format: Article
• Language: English
• Subjects: Agricultural sciences; Biochemistry, Molecular Biology; Biomass; Cell Wall - drug effects; Cell Wall - metabolism; co‐regulation networks; drought; Eucalyptus; Eucalyptus - drug effects; Eucalyptus - growth & development; Gene Regulatory Networks - drug effects; Genetics; Genomics; Life Sciences; Metabolome - drug effects; omics integration; Phenotype; Plant Proteins - metabolism; Plants genetics; potassium; Potassium - pharmacology; Silviculture, forestry; Systems Biology; transcription factors; Transcription Factors - metabolism; Transcriptome - genetics; Trees - drug effects; Trees - growth & development; Water - pharmacology; Wood - drug effects; Wood - growth & development; xylem; Xylem - drug effects; Xylem - genetics; Xylem - growth & development
• ISSN: 0028-646X; 1469-8137
• DOI: 10.1111/nph.15802

Wood production in fast-growing Eucalyptus grandis trees is highly dependent on both potassium (K) fertilization and water availability but the molecular processes underlying wood formation in response to the combined effects of these two limiting factors remain unknown. E. grandis trees were submitted to four combinations of K-fertilization and water supply. Weighted gene co-expression network analysis and MixOmics-based co-regulation networks were used to integrate xylem transcriptome, metabolome and complex wood traits. Functional characterization of a candidate gene was performed in transgenic E. grandis hairy roots. This integrated network-based approach enabled us to identify meaningful biological processes and regulators impacted by K-fertilization and/or water limitation. It revealed that modules of co-regulated genes and metabolites strongly correlated to wood complex traits are in the heart of a complex trade-off between biomass production and stress responses. Nested in these modules, potential new cell-wall regulators were identified, as further confirmed by the functional characterization of EgMYB137. These findings provide new insights into the regulatory mechanisms of wood formation under stressful conditions, pointing out both known and new regulators co-opted by K-fertilization and/or water limitation that may potentially promote adaptive wood traits.