Migration of Deformable Vesicles Induced by Ionic Stimuli

• Published in: Langmuir 2018-09, Vol.34 (38), p.11484-11494
• Main Authors: Kodama, Atsuji, Morandi, Mattia, Ebihara, Ryuta, Jimbo, Takehiro, Toyoda, Masayuki, Sakuma, Yuka, Imai, Masayuki, Puff, Nicolas, Angelova, Miglena I
• Format: Article
• Language: English
• Subjects: Biological Physics; Physics
• ISSN: 0743-7463; 1520-5827
• DOI: 10.1021/acs.langmuir.8b02105

We have investigated the dynamics of phospholipid vesicles composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine triggered by ionic stimuli using electrolytes such as CaCl2, NaCl, and NaOH. The ionic stimuli induce two characteristic vesicle dynamics, deformation due to the ion binding to the lipids in the outer leaflet of the vesicle and migration due to the concentration gradient of ions, that is, diffusiophoresis or the interfacial energy gradient mechanism. We examined the deformation pathway for each electrolyte as a function of time and analyzed it based on the surface dissociation model and the area difference elasticity model, which reveals the change of the cross-sectional area of the phospholipid by the ion binding. The metal ions such as Ca2+ and Na+ encourage inward budding deformation by decreasing the cross-sectional area of a lipid, whereas the hydroxide ion (OH–) encourages outward budding deformation by increasing the cross-sectional area of a lipid. When we microinjected these electrolytes toward the vesicles, a strong coupling between the deformation and the migration of the vesicle was observed for CaCl2 and NaOH, whereas for NaCl, the coupling was very weak. This difference probably originates from the binding constants of the ions.