Application of Western blot analysis for the diagnosis of Encephalitozoon cuniculi infection in rabbits: example of a quantitative approach

Diagnosis of Encephalitozoon cuniculi infection in rabbits remains a major veterinary issue. ELISA or immunofluorescence assays are the current reference standards of serological tests. However, these conventional techniques suffer from a lack of accuracy for distinguishing active from past infectio...

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Bibliographic Details
Published in:Parasitology research (1987) 2017-02, Vol.116 (2), p.743-750
Main Authors: Desoubeaux, Guillaume, Pantin, Ana, Peschke, Roman, Joachim, Anja, Cray, Carolyn
Format: Article
Language:English
Subjects:
Animals
Biomedical and Life Sciences
Biomedicine
Blotting, Western - methods
Blotting, Western - veterinary
Diagnosis
Encephalitozoon cuniculi - immunology
Encephalitozoon cuniculi - isolation & purification
Encephalitozoonosis - diagnosis
Encephalitozoonosis - microbiology
Encephalitozoonosis - veterinary
Enzyme-linked immunosorbent assay
Enzyme-Linked Immunosorbent Assay - veterinary
Female
Fluorescent Antibody Technique, Direct - veterinary
Health aspects
Human health and pathology
Immune response
Immune response (humoral)
Immunofluorescence
Immunoglobulin G
Immunoglobulin G - blood
Immunoglobulin M
Immunoglobulin M - blood
Immunoglobulins
Immunology
Infection
Infections
Infectious diseases
Life Sciences
Male
Medical colleges
Medical Microbiology
Methods
Microbiology
Microbiology and Parasitology
Mycology
Original Paper
Parasitology
Rabbits - microbiology
Serological tests
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Summary:Diagnosis of Encephalitozoon cuniculi infection in rabbits remains a major veterinary issue. ELISA or immunofluorescence assays are the current reference standards of serological tests. However, these conventional techniques suffer from a lack of accuracy for distinguishing active from past infections, as a positive serostatus is common in clinically normal rabbits. In this study, we assessed the diagnostic performance of Western blot (WB) to detect both anti- E. cuniculi immunoglobulin G (IgG) and immunoglobulin M (IgM) in comparison with ELISA and to address the intensity of the immune response through a quantitative approach. Positive WB results were highly correlated with the E. cuniculi -related diseased status ( P  
ISSN:0932-0113
1432-1955
DOI:10.1007/s00436-016-5343-4