Electromigration separation methodologies for the characterization of nanoparticles and the evaluation of their behaviour in biological systems

•CE shows high resolution, short analysis time, low sample consumption and mimics in vivo conditions.•Physicochemical parameters can be accurately obtained from mobility by using mathematical models.•Characterization of NPs in biological conditions helps for their design for biomedical purpose.•CE h...

Full description

Saved in:
Bibliographic Details
Published in:TrAC, Trends in analytical chemistry (Regular ed.) Trends in analytical chemistry (Regular ed.), 2016-11, Vol.84, p.121-130
Main Authors: Trapiella-Alfonso, Laura, Ramírez-García, Gonzalo, d'Orlyé, Fanny, Varenne, Anne
Format: Article
Language:English
Subjects:
Analytical chemistry
Biocompatibility
Biomedical applications
Biomolecule interactions
Capillary electrophoresis
Chemical Sciences
Nanoparticles
Physicochemical characterization
Physiological media
Protein corona
Taylor dispersion analysis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:•CE shows high resolution, short analysis time, low sample consumption and mimics in vivo conditions.•Physicochemical parameters can be accurately obtained from mobility by using mathematical models.•Characterization of NPs in biological conditions helps for their design for biomedical purpose.•CE has demonstrated to be indispensable for the evaluation of NP-biomolecule interactions.•Coupling CE with sensitive detectors is a way to improve the NPs characterization The use of nanoparticles (NPs) for biomedical applications implies the knowledge of different physicochemical parameters in physiologically relevant media. Electromigration separation methods have demonstrated to be powerful analytical tools for the separation and whole characterization of such NPs as well as to help in the design and control of NPs synthesis and surface modification processes. The different working modes exploited at this date to obtain complementary information about colloidal NP properties such as size, ζ-potential, surface charge density, grafting rates and binding parameters as well as their colloidal stability in different media are reviewed in this work. We put particular emphasis on the electromigration separation methods developed for NPs used in biomedical sciences that help to predict the behaviour of such objects in the biological systems. Finally some perspectives and research lines envisaged in this domain are proposed.
ISSN:0165-9936
1879-3142
0165-9936
DOI:10.1016/j.trac.2016.04.022