Integrated device for combined optical neuromodulation and electrical recording for chronic in vivo applications

Studying brain function and its local circuit dynamics requires neural interfaces that can record and stimulate the brain with high spatiotemporal resolution. Optogenetics, a technique that genetically targets specific neurons to express light-sensitive channel proteins, provides the capability to c...

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Bibliographic Details
Published in:Journal of neural engineering 2012-02, Vol.9 (1), p.016001-016001
Main Authors: Wang, Jing, Wagner, Fabien, Borton, David A, Zhang, Jiayi, Ozden, Ilker, Burwell, Rebecca D, Nurmikko, Arto V, van Wagenen, Rick, Diester, Ilka, Deisseroth, Karl
Format: Article
Language:English
Subjects:
Action Potentials - physiology
Animals
Brain - physiology
Electrodes, Implanted
Electroencephalography - instrumentation
Equipment Design
Equipment Failure Analysis
Fiber Optic Technology - instrumentation
Life Sciences
Male
Monitoring, Ambulatory - instrumentation
Neurobiology
Neurons - physiology
Neurons and Cognition
Rats
Systems Integration
Voltage-Sensitive Dye Imaging - instrumentation
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Summary:Studying brain function and its local circuit dynamics requires neural interfaces that can record and stimulate the brain with high spatiotemporal resolution. Optogenetics, a technique that genetically targets specific neurons to express light-sensitive channel proteins, provides the capability to control central nervous system neuronal activity in mammals with millisecond time precision. This technique enables precise optical stimulation of neurons and simultaneous monitoring of neural response by electrophysiological means, both in the vicinity of and distant to the stimulation site. We previously demonstrated, in vitro, the dual capability (optical delivery and electrical recording) while testing a novel hybrid device (optrode-MEA), which incorporates a tapered coaxial optical electrode (optrode) and a 100 element microelectrode array (MEA). Here we report a fully chronic implant of a new version of this device in ChR2-expressing rats, and demonstrate its use in freely moving animals over periods up to 8 months. In its present configuration, we show the device delivering optical excitation to a single cortical site while mapping the neural response from the surrounding 30 channels of the 6 × 6 element MEA, thereby enabling recording of optically modulated single-unit and local field potential activity across several millimeters of the neocortical landscape.
ISSN:1741-2560
1741-2552
DOI:10.1088/1741-2560/9/1/016001